Bio-Rad 2150-0180 Collagen V,Rabbit anti Human Collagen V

2024-10-22

Rabbit anti Human Collagen V

货号:2150-0180

规格:0.5 ml

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Collagen V

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human collagen V atibodyrecognizes human collagen V and demonstrates th following cross reactivities as demonstrated by ELISA:Human collagen type V 100%Human collagen type IV <5%Human collagen type I & III <3%Human plasma proteins <0.5%
产品详情

Target Species

Human

Product Form

Purified IgG - lyophilized

Preparation

Purified IgG prepared by affinity chromatography

Buffer Solution

0.15M Sodium Chloride0.1M Sodium Phosphate

Preservative Stabilisers

1% Mannitol1% Dextran

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml after reconstitution

Immunogen

Native human collagen V from placenta.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

Reconstitution

Reconstitute with 0.5 ml distilled water containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
存储条件
Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/1500

Immunofluorescence

Immunohistology - Paraffin11/20

Immunohistology - Frozen

1/20
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

1This product requires protein digestion pre-treatment of paraffin sections. 1 mg/ml pepsin in 0.5M acetic acid for 2 hours at 37ºC and multiple buffer washes is recommended.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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文献

1. Evans, M. J.et al.(2002) Fibroblast growth factor-2 during postnatal development of the tracheal basement membraneAm J Physiol Lung Cell Mol Physiol 283: 1263 - 12702. Mohan, H.et al.(2010) Extracellular matrix in multiple sclerosis lesions: Fibrillar collagens, biglycan and decorin are upregulated and associated with infiltrating immune cells.Brain Pathol. 20: 966-75.

技术参数

产品应用 IHC(F);ELISA;IF;IHC(P)*

产品类型 Polyclonal Antibody

推荐用于 IHC(F);ELISA;IF;IHC(P)*

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