Bio-Rad Angiotensin I抗体 0500-0011,Mouse anti Human Angiotensin I

2024-10-22

Mouse anti Human Angiotensin I

货号:0500-0011

规格:0.2 mg

价格:3043

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Angiotensin I

物种:人

宿主:小鼠

抗体亚型:IgG2b

克隆号:13/96/Ang C9 (BGN/KA/22H)

荧光染料:Purified

产品介绍

Mouse anti Human angiotensin I antibody, clone 13/96/Ang C9recognizes angiotensin I, a 10 amino acid peptide derived by the proteolytic processing of angiotensinogen. Angiotensin I is important in the Renin-Angiotensin-Aldosterone System (RAAS). RAAS is responsible for the regulation of blood pressure and water retention in the body.Angiotensin I is produced from a reaction between the renal enzyme Renin and angiotensinogen. It is further converted by Angiotensin Converting Enzyme (ACE) into a vasoactive form called Angiotensin II which is responsible for increasing blood pressure. Mouse anti Human angiotensin I antibody does not react with angiotensin II, III or angiotensinogen in ELISA.

产品详情

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

Immunogen

HMW conjugate.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
  • ELISA
  • In a simple indirect ELISA, using 1.0 µg of Angiotensin I coated per well, the 50% binding was observed at an antibody dilution of 1/950. Under the same conditions, the end point titre was observed at an antibody dilution of 1/10K.

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文献

1.Luna-Vital, D.A.et al.(2016) Dietary peptides from the non-digestible fraction ofPhaseolus vulgarisL. decrease angiotensin II-dependent proliferation in HCT116 human colorectal cancer cells through the blockade of the renin-angiotensin system.Food Funct. 7 (5): 2409-19.

技术参数

产品应用 ELISA

产品类型 Monoclonal Antibody

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