| 产品介绍 | | |
| Mouse anti Human C3b antibody, clone H11recognizes human C3b, part of the complement system. C3 is cleaved by C3-convertase creating C3a and C3b, which binds to the surface of pathogens leading to their destruction by phagocytic cells. Mouse anti Human C3b antibody, clone H11 recognizes a determinant present on native (circulating) C3 and on the fragments C3b and C3c. The antibody is directed to a determinant present on the betachain of the C3 molecule (Western blot). It does not react with the anaphylatoxin C3a or the fragment C3d and does not inhibit the haemolytic function of C3. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - lyophilized |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide, 0.5% Bovine Serum Albumin.These concentrations relate to the reconstituted product. |
Approx. Protein Concentrations | IgG concentration 50 ug/ml after reconstitution |
Immunogen | Human C3b. |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
Reconstitution | Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gentle mixing of the vial following reconstitution is recommended. |
| 存储条件 | | |
| This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | | |
Immunohistology - Frozen | √ | | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
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| 数据 |
Published customer image:Mouse anti Human C3b antibody, clone H11 (0200-0614) used for the evaluation of C3b expression on CD59 positive and negative populations of human erythrocytes by flow cytometry.Image caption:In vivoC3 binding on red cells of PNH patients on eculizumab. a Kinetics of C3 binding on red cells from a representative PNH patient during eculizumab treatment. Before treatment (0 week), no red cell binds C3, whereas starting from 1 week of treatment three populations of red cells are displayed: one population of normal red cells (CD59 + C3−) and two distinct populations of PNH (CD59-negative) red cells, one with (C3+) and one without (C3−) fragments bound on their surface. b Characterization of C3 fragment bound to erythrocytes from PNH patient on eculizumab. The left panel shows the normal (CD59-positive) and PNH (CD59-negative) populations (gated by the elliptic mark). The PNH (CD59-negative) population (see gate in the left panel) has been analyzed with anti-C3d and either anti-C3b (middle panel) or anti-iC3b (left panel). SSC: side scatterFrom: Sica M, Rondelli T, Ricci P, De Angioletti M, Risitano AM, Notaro R.Eculizumab treatment: stochastic occurrence of C3 binding to individual PNH erythrocytes.J Hematol Oncol. 2017 Jun 19;10(1):126. |
| 文献 |
| 1. Wörner, I.et al.(1989) Localization and functional charaterization of epitopes on α and β-chains of C3Complement Inflamm: Abstracts Xlllth International Complement Workshop 6: 416.References for C3b antibody2. Hallström, T.et al.(2011) Immune Evasion ofMoraxella catarrhalisInvolves Ubiquitous Surface Protein A-Dependent C3d Binding.J Immunol. 186: 3120-9.3. Sica, M.et al.(2017) Eculizumab treatment: stochastic occurrence of C3 binding to individual PNH erythrocytes.J Hematol Oncol. 10 (1): 126. |
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