Bio-Rad Osteocalcin抗体 0400-0041,Mouse anti Human Osteocalcin

2024-10-22

Mouse anti Human Osteocalcin

货号:0400-0041

规格:0.2 mg

价格:3043

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Osteocalcin

物种:人

宿主:小鼠

抗体亚型:IgG2a

克隆号:2H9F11F8

荧光染料:Purified

产品介绍

Mouse anti Human Osteocalcin antibody, clone 2H9F11F8recognizes intact human and bovine osteocalcin in a "2 site" assay and reacts with osteocalcin fragments corresponding to amino acids 15-31 and 20-43 of the native molecule. It does not recognize fragments representing amino acids 1-19 or 7-19.Osteocalcin (Bone Gla Protein) is a 49 amino acid single chain vitamin K dependent protein (MW 5.8 kDa), made by osteoblasts that is a major component of the non-collagenous bone matrix. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget’s disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency.

产品详情

Target Species

Human

Species Cross-Reactivity

Target Species

CrossReactivity

Rabbit

Pig

Mouse

×

Rat

Bovine

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%Sodium Azide (NaN3)

Carrier Free

Yes

Immunogen

Recombinant human osteocalcin GST fusion protein.

Approx. Protein Concentrations

1.0 mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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文献

1.Rehder, D.S.et al.(2015) Gamma-carboxylation and fragmentation of osteocalcin in human serum defined by mass spectrometry.Mol Cell Proteomics. 14 (6): 1546-55.2.Käkönen, S-M.et al.(2005) Method for prediction of bone fractures by osteocalcin measurementsUS Patent: US6967081B1

技术参数

产品应用 Elisa

产品类型 Monoclonal Antibody

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