Mouse anti Human Interleukin-4 Receptor antibody, clone 2546,Mouse anti Human Interleukin-4 Receptor antibody, clone 25463

2024-10-22

Mouse anti Human Interleukin-4 Receptor antibody, clone 25463

货号:0100-0462

规格:0.1 mg

价格:4888

产品类型:免疫组化一抗

品牌:Bio-Rad

物种:人

宿主:小鼠

抗体亚型:IgG2a

荧光染料:Purified

产品介绍
Mouse anti Human Interleukin-4 Receptor antibody, clone 25463recognizes human Interleukin-4 receptor subunit alpha, also known as CD124. CD124 is a 825 amino acid ~90kDa type I single pass transmembrane glycoprotein bearing a singlefibronectin type IIIdomain. Interleukin-4 Receptor form a heterodimeric complex with thecytokine receptor common subunit gamma, referred to as the IL-4 type-1 complex. The alpha subunit can also form the type-II complex through association with the IL-13 receptor alpha 1 subunit. The IL-4R complex acts as a receptor for both interleukin-4 and interleukin-13. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.Mouse anti Human Interleukin-4 Receptor antibody, clone 25463 is reported to block human cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50was observed at approximately 5-20 ng/ml in the presence of 0.2 ng/ml of IL-4 using the factor dependant human erythroleukemic cell line, TF-1.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from ascites
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.5% Trehalose
ImmunogeSpodoptera frugiperdalepidopteran ovarian cell lineSf21-derived, recombinant human interleukin 4 receptor, extracellular domain.
Approx. Protein ConcentrationsIgG concentration 0.5 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Store at -20°C only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA1/2501/1000
Flow Cytometry1/101/40
Immunohistology - Paraffin1/201/60
Western Blotting1/1251/500
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117FF0.5mg
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117AE,WB0.5mg

技术参数

产品应用 Elisa

产品类型 Monoclonal Antibody

推荐用于 Elisa

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