Bio-Rad 740605,Recombinant Protein G:HRP

2024-10-23

Recombinant Protein G:HRP

货号:740605

规格:1 ml

价格:2406

产品类型:蛋白

品牌:Bio-Rad

产品介绍

Recombinant Protein Gis a preparation of streptococcal protein G expressed inE. coli. Protein G binds to the Fc portion of immunoglobulin G subclasses (IgGs) from a broad range of species including human and mouse, and does not bind IgA, IgE, IgM or serum albumin.

产品详情

Target Species

Bacterial

Product Form

Recombinant protein - conjugated to Horseradish Peroxidase (HRP) - liquid

Preparation

Recombinant protein G fromStreptococcusstrain G148, expressed inE.coli.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

50% Glycerol

Protein Molecular Weight

22 kDa (monomeric protein lacking cystine residues)

Regulatory

For research purposes only

Guarantee

Guaranteed until date of expiry. Please see product label.

存储条件

Store at -20oC only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA1/40001/8000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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文献

1.Saegerman et al (2004) Evalution of three serum i-ELISAs using monoclonal antibodies and protein G as peroxidase conjugate for the diagnosis of bovine brucellosis.Vet Microbiol. 20. 91-105.2.Qi et al (2001) Chromatography on DEAE ion-exchange and Protein G affinity columns in tandem for the separation and purification of proteins.Biochem Biophys Methods. 49. 263-273.3.McDonald, J.U.et al.(2016) Development of a custom pentaplex sandwich immunoassay using Protein-G coupled beads for the Luminex® xMAP® platform.J Immunol Methods. pii: S0022-1759(16)30033-3. [Epub ahead of print]

技术参数

产品应用 ELISA

产品类型 Recombinant Protein

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