Bio-Rad IgG抗体 643001,Donkey anti Rat IgG (H/L) (Mouse Adsorbed)

2024-10-23

Donkey anti Rat IgG (H/L) (Mouse Adsorbed)

货号:643001

规格:1 mg

价格:2312

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:IgG

物种:大鼠

宿主:驴

抗体亚型:IgG

荧光染料:Purified

产品介绍

Donkey anti Goat IgG antibodyrecognizes the heavy and light chains of goat IgG as demonstrated by ELISA. The preparation has minimal cross reaction with rat and mouse serum proteins, however, it may react with the light chains of other goat immunoglobulins.

产品详情

Target Species

Goat

Species Cross-Reactivity

TargetSpecies

CrossReactivity

Mouse

×

Rat

×

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid

Antiserum Preparation

Antiserum to goat IgG (H/L) was raised by repeated immunisation of donkeys with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN3)Stabilizing agent (sucrose)

Immunogen

Goat IgG.

Approx. Protein Concentrations

0.5 mg/ml

Max Ex/Em

Fluorophore

Excitation Max (nm)

Emission Max (nm)

RPE 488nm laser

496

578

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

+4ºC DO NOT FREEZEThis product is photosensitive and should be protected from light.

应用

Application Name

Verified

Min Dilution

Max Dilution

Flow Cytometry

1/10

1/50

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry
  • Use 10ul of the suggested working dilution to label 1x106cells in 100ul

文献

1.Gerry, A.B. and Leake, D.S. (2014) Effect of low extracellular pH on NF-κB activation in macrophages.Atherosclerosis. 233: 537-44.2.Armour, K.L.et al.(2014) Clearance of Human IgG1-Sensitised Red Blood Cells In Vivo in Humans Relates to the In Vitro Properties of Antibodies from Alternative Cell Lines.PLoS One. 9: e109463.

技术参数

产品应用 C ;E

产品类型 Polyclonal Antibody

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