| 产品介绍 | | |
| Mouse IgG1 Negative Control antibodyis suitable for use as a negative control to assess non-specific binding of mouse IgG1 antibodies to target cells. Mouse IgG1 Negative Control antibody has been tested and found to be negative on the following rat cell types, peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Clone F8-11-13 recognises the human CD45RA antigen, and therefore human leucocytes may be used as a positive control for this product. NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES |
| 产品详情 | | |
Target Species | Negative Control |
Product Form | Purified IgG conjugated to Alexa Fluor® 647 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1%Bovine Serum Albumin |
Immunogen | Human T lymphocytes. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®647 | 650 | 665 |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.Use 10ul of the suggested working dilution to label 106cells in 100ul |
| 数据 |
| Figure A. FITC conjugated mouse anti rat CD45RA (MCA340FT) and A647 conjugated mouse IgG1 isotype control (MCA1209A647). Figure B. FITC conjugated mouse anti rat CD45RA (MCA340FT) and A647 conjugated mouse anti rat CD45 (MCA43A647). All experiments performed on red cell lysed rat blood gated on mononuclear cells. |
| 文献 |
| 1. Weiss, D.J.et al.(2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate ofMycobacterium aviumsubsp.paratuberculosisandMycobacterium aviumsubsp.avium.J Leukoc Biol. 83 (1): 48-55.2. Chen, W.et al.(2009) Expression of toll-like receptor 4 in uvea-resident tissue macrophages during endotoxin-induced uveitis.Mol Vis. 15: 619-28.3. Safeukui Iet al.(2015) Malaria induces anemia through CD8+ T cell-dependent parasite clearance and erythrocyte removal in the spleen.MBio. 6 (1) pii: e02493-14.4. Aricha, R.et al.(2016) Suppression of experimental autoimmune myasthenia gravis by autologous T regulatory cells.J Autoimmun. 67: 57-64.5. Wattegedera, S.R.et al.(2017) Enhancing the toolbox to study IL-17A in cattle and sheep.Vet Res. 48 (1): 20.6. Stangl, H.et al.(2020) MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.Sci Rep. 10 (1): 17071. |
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