Bio-Rad MCA1209B 小鼠同型,Mouse IgG1 Negative Control:Biotin-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1209B 小鼠同型,Mouse IgG1 Negative Control:Biotin

2024-10-23

Mouse IgG1 Negative Control:Biotin

货号：MCA1209B

规格：0.1 mg

价格：2695

产品类型：小鼠同型对照

品牌：Bio-Rad

物种：人

宿主：小鼠

抗体亚型：IgG1

荧光染料：Biotin

产品介绍
Mouse IgG1 Negative Control antibodyis suitable for use as a negative control to assess non-specific binding of mouse IgG1 antibodies to target cells. Mouse IgG1 Negative Control antibody has been tested and found to be negative on the following rat cell types, peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Clone F8-11-13 recognises the human CD45RA antigen, and therefore human leucocytes may be used as a positive control for this product. NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES
产品详情
Target Species	Negative Control
Product Form	Purified IgG conjugated to Biotin - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1%Bovine Serum Albumin
Immunogen	Human T lymphocytes.
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶cells in 100ul
文献
1. Weiss, D.J.et al.(2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate ofMycobacterium aviumsubsp.paratuberculosisandMycobacterium aviumsubsp.avium.J Leukoc Biol. 83 (1): 48-55.2. Chen, W.et al.(2009) Expression of toll-like receptor 4 in uvea-resident tissue macrophages during endotoxin-induced uveitis.Mol Vis. 15: 619-28.3. Safeukui Iet al.(2015) Malaria induces anemia through CD8+ T cell-dependent parasite clearance and erythrocyte removal in the spleen.MBio. 6 (1) pii: e02493-14.4. Aricha, R.et al.(2016) Suppression of experimental autoimmune myasthenia gravis by autologous T regulatory cells.J Autoimmun. 67: 57-64.5. Wattegedera, S.R.et al.(2017) Enhancing the toolbox to study IL-17A in cattle and sheep.Vet Res. 48 (1): 20.6. Stangl, H.et al.(2020) MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.Sci Rep. 10 (1): 17071.

技术参数

产品应用 Flow

产品类型 Negative/Isotype Control

推荐用于 Flow

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Bio-Rad MCA1209B 小鼠同 型,Mouse IgG1 Negative Control:Biotin

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