TRA-1-60 Monoclonal Antibody (TRA-1-60), DyLight 550

货号：MA1-023-D550

规格：100μl

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号： TRA-1-60

荧光染料：DyLight 488

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1:10-1:100(ICC/IF)

产品详细信息MA1-023-D550 has been successfully used in ICC/IF applications on human samples.靶标信息TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

数据

TRA-1-60 Antibody (MA1-023-D550) in ICC/IFImmunofluorescent analysis of TRA-1-60 (red) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 550-conjugated TRA-1-60 monoclonal antibody (Product # MA1-023-D550) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. Nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.

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