Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)), Biotin

货号：MA511544

规格：500 µL

价格：5442

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人/小鼠/非人灵长类/猪/恒河猴

宿主：小鼠

抗体亚型：IgG2a

克隆号：1A4 (asm-1)

荧光染料：Biotin

类型:	单抗	同型对照：
浓度：	40 µg/mL	用法：	1:100-1:500 (WB)；1:800 (IHC (P))；Assay-dependent(ICC/IF)

产品详细信息MA5-11544 targets Actin Smooth Muscle in WB, immunofluorescence and immunohistochemistry applications and shows reactivity with cow, chicken, human, mouse, non-human primate, rabbit, and rat samples.The MA5-11544 immunogen is n-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus.靶标信息Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

数据

Alpha-Smooth Muscle Actin Antibody (MA5-11544) in WBWestern blot analysis of Actin Sooth Muscle was performed by loading 25 µg of C2C12 (Lane 1), Hela (Lane 2), and A431 (Lane 3) cell lysates and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4°C overnight. The membrane was probed with an Actin Sooth Muscle monoclonal antibody (Product # MA5-11547) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Results show a band at 42 kDa in all three cell lysates.

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参考文献：

1.PloS oneAttenuation of CCl4-induced hepatic fibrosis in mice by vaccinating against TGF-β1."MA5-11544 was used in western blot to study the ability of vaccination against TGF-beta1 to protect against hepatic fibrosis in a liver injury model"2.OncogeneGermline mutation of Brca1 alters the fate of mammary luminal cells and causes luminal-to-basal mammary tumor transformation."MA5-11544 was used in western blot to study the mechanism by which germline Brca1 mutation promotes luminal-to-basal mammary tumor transformation"3.The Journal of pharmacology and experimental therapeuticsVaccination with platelet-derived growth factor B kinoids inhibits CCl₄-induced hepatic fibrosis in mice."MA5-11544 was used in western blot to study the ability of vaccination against PDGF-D to protect against hepatic fibrosis in a rat model of acute liver injury"4.Matrix biology : journal of the International Society for Matrix BiologyTight skin 2 mice exhibit a novel time line of events leading to increased extracellular matrix deposition and dermal fibrosis."MA5-11544 was used in immunohistochemistry to study the time-course of extracellular matrix deposition and dermal fibrogenesis in the murine Tsk2 model of systemic sclerosis"5.Laboratory investigation; a journal of technical methods and pathologyDuctular reactions in the liver regeneration process with local inflammation after physical partial hepatectomy."MA511544 was used in immunohistochemistry - paraffin section to examine the liver regeneration process after physical partial hepatectomy"

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