alpha-1 Antitrypsin Monoclonal Antibody (2B12)

货号：MA5-15521

规格：100 µL

价格：5463

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1

克隆号： 2B12

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1:500-1:2,000 (WB)；1:10,000(ELISA)

产品详细信息MA5-15521 targets AAT in WB applications and shows reactivity with Human and mouse samples.The MA5-15521 immunogen is purified recombinant fragment of human AAT expressed in E. Coli.MA5-15521 detects AAT which has a predicted molecular weight of approximately 47kDa.靶标信息Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.

数据

alpha-1 Antitrypsin Antibody (MA5-15521) in WBWestern blot was performed using Anti-alpha-1 Antitrypsin Monoclonal Antibody (2B12)(Product # MA5-15521) and a ~55kDa band corresponding to alpha-1 Antitrypsin was observed across cell lines. Whole Cell Extract-TEB (30 µg lysate) of Hep G2 (Lane 1) and HT-29 (Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Scientific reportsImproved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase."MA515521 was used in western blot to optimize bi-allelic modification of transcriptionally silent loci in human pluripotent stem cells"

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-44026.htm