Human Serum Albumin Monoclonal Antibody (1A9)

货号：MA120125

规格：100 µg

价格：4786

产品类型：抗体和染料

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG2a

克隆号：1A9

荧光染料：其它

类型:	单抗	同型对照：
浓度：	6.5 mg/mL	用法：	Assay-dependent (ELISA)；Assay-dependent免疫沉淀 (IP)；Assay-dependent放射免疫测定 (RIA)

产品详细信息MA1-20125 detects albumin in human samples.靶标信息Albumin is a soluble, monomeric protein with a molecular weight of approximately 65-67 kDa that comprises about one-half of the blood serum protein. Human serum albumin (HAS) is the most abundant protein in human blood plasma, and has a serum half-life of approximately 20 days. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular, unglycosylated serum protein synthesized in the liver as pre-pro-albumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough `produce the secreted albumin. Serum albumin has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. The main function of albumin is the regulation of the colloidal osmotic pressure of blood.

数据

Human Serum Albumin Antibody (MA1-20125) in WBWestern blot was performed using Anti-Human Serum Albumin Monoclonal Antibody (1A9)(Product # MA1-20125) and a 66kDa band corresponding to Human Serum Albumin was observed in Hep G2 when compared to HCT 116 which is reported to be a low expressing model. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with PTI (1X for 4hrs (Lane 2), HCT 116 (Lane 3), HCT 116 treated with PTI (1X for 4hrs) (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

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