CXCR4 Monoclonal Antibody (12G5)

货号：35-8800

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：12G5

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	3-5 µg/mL(Flow)

产品详细信息By Western blot, 35-8800 detects a band at ~60kD, which likely represents an isoform or post-translationally modified form of CXCR4, many of which have been described in the literature.靶标信息CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

数据

CXCR4 Antibody (35-8800) in WBWestern blot analysis of CXCR4 was performed by loading 25 µg of Ramos, Raji, and Jurkat whole cell lysates, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Blotter (Product # 62288), and blocked with 5% milk in TBST for at least 1 hour at room temperature. CXCR4 was detected at ~60 kD using a CXCR4 monoclonal antibody at a concentration of 2 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:40,000 for at least 30 minutes. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076). NOTE: The presence of a 60 kD band likely represents an isoform or post-translationally modified form of CXCR4, many of which have been described in the literature.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Biological & pharmaceutical bulletinMiR-140-3p Ameliorates the Progression of Osteoarthritis via Targeting CXCR4."35-8800 was used in Western Blotting to evaluate the potential function of miRNA-140-3p during the pathogenesis of osteoarthritis."2.CancersCocktail Strategy Based on NK Cell-Derived Exosomes and Their Biomimetic Nanoparticles for Dual Tumor Therapy."35-8800 was used in Western Blotting to propose this NN/NKEXO cocktail as a new avenue for tumor therapy, with potential prospects for clinical applications."3.Oncology reportsHypoxia induces CXCR4 expression and biological activity in gastric cancer cells through activation of hypoxia-inducible factor-1α."35-8800 was used in Western Blotting to study the role of hypoxia in regulating CXCR4 expression and biological activity in gastric cancer."4.Pathology oncology research : PORImmunohistochemical Predictors of Bone Metastases in Breast Cancer Patients."35-8800 was used in immunohistochemistry to evaluate the potential protein markers of bone metastases in breast cancer patients."5.World journal of hepatologyParticipation of peribiliary glands in biliary tract pathophysiologies."Published figure using CXCR4 monoclonal antibody (Product # 35-8800) in Immunohistochemistry"

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-44043.htm