c-Kit Polyclonal Antibody

货号：34-8800

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.25 mg/mL	用法：	Assay-dependent(WB)；1:10-1:50 (IHC (P))；2 µg/mL(ICC/IF)

靶标信息c-Kit, also known as mast/stem cell growth factor receptor (SCFR) or CD117, is a trans membrane type III tyrosine kinase receptor encoded by the c-Kit proto-oncogene, located on chromosome 4 in humans. It is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

数据

c-Kit Antibody (34-8800) in ICC/IFImmunofluorescence analysis of c-Kit/CD117 was performed using 70% confluent log phase SH-SY5Y cells treated with 10 uM retinoic acid for 72 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with c-Kit/CD117 Rabbit Polyclonal Antibody (Product # 34-8800) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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参考文献：

1.ProtoplasmaTelocytes in the mouse testicular interstitium: implications of G-protein-coupled estrogen receptor (GPER) and estrogen-related receptor (ERR) in the regulation of mouse testicular interstitial cells."34-8800 was used in Immunohistochemistry to study telocytes as a target cell type for the prevention and treatment of testicular interstitial tissue disorders based on disturbances in oestrogen-signalling."2.Cancer researchHypoxia-regulated delta-like 1 homologue enhances cancer cell stemness and tumorigenicity.AuthorsKim Y,Lin Q,Zelterman D,Yun Z3.Scientific reportsProtective effect of thymoquinone against lung intoxication induced by malathion inhalation."34-8800 was used in Immunohistochemistry (Paraffin) to show thymoquinone could be potentially used in pest control workers to ameliorate the systemic and pulmonary intoxication caused by one of the most field-used pesticides."4.ProtoplasmaTelocytes in the mouse testicular interstitium: implications of G-protein-coupled estrogen receptor (GPER) and estrogen-related receptor (ERR) in the regulation of mouse testicular interstitial cells."34-8800 was used in Immunohistochemistry to study telocytes as a target cell type for the prevention and treatment of testicular interstitial tissue disorders based on disturbances in oestrogen-signalling."5.Scientific reportsProtective effect of thymoquinone against lung intoxication induced by malathion inhalation."34-8800 was used in Immunohistochemistry (Paraffin) to show thymoquinone could be potentially used in pest control workers to ameliorate the systemic and pulmonary intoxication caused by one of the most field-used pesticides."

技术参数

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