Chromogranin A Monoclonal Antibody (LK2H10)

货号：MA5-13096

规格：500 µL

价格：4130

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.2 mg/mL	用法：	1-2 µg/mL(WB)；1:800 (IHC (P))；1:10-1:100(ICC/IF)

产品详细信息MA5-13096 targets Chromogranin A in IHC (P), ICC/IF and WB applications and shows reactivity with Human, Non-human primate, Porcine, Rabbit, and Rat samples.The MA5-13096 immunogen is human pheochromocytoma.靶标信息Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocine cells. CHGA is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides act as autocine or paracrine negative modulators of the neuroendocrine system. CHGA is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

数据

Chromogranin A Antibody (MA5-13096) in ICC/IFImmunofluorescent analysis of Chromogranin A (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Chromogranin A monoclonal antibody (Product # MA5-13096) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x

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参考文献：

1.Oncotargetc-MYC drives histone demethylase PHF8 during neuroendocrine differentiation and in castration-resistant prostate cancer."MA513096 was used in western blot to elucidate the regulation of PHF8 and KDM3A during neuroendocrine differentiation and castration-resistant prostate cancer"2.Regulatory peptidesChromogranin peptides in amyotrophic lateral sclerosis."MA5-13096 was used in western blot to investigate the role of chromogranin peptides in amyotrophic lateral sclerosis"3.Cancer researchTwo functional epitopes of pigment epithelial-derived factor block angiogenesis and induce differentiation in prostate cancer."MA5-13096 was used in western blot to investigate the role of two functional epitopes of pigment epithelial-derived factor in prostate cancer"4.EndocrinologyHeparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function."MA5-13096 was used in western blot to investigate the influence of heparin-binding epidermal growth factor-like growth factor on prostate tumor growth"5.Journal of visualized experiments : JoVEEstablishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids."MA5-13096 was used in Immunohistochemistry-immunofluorescence to characterise SBNET markers."AuthorsEar PH,Li G,Wu M,Abusada E,Bellizzi AM,Howe JR

技术参数

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