Chromogranin A Monoclonal Antibody (PHE5)

货号：MA5-13281

规格：500 µL

价格：4070

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG1

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.2 mg/mL	用法：	0.5-1.0 µg/mL(IHC (P))

产品详细信息MA5-13281 targets Chromogranin A in IF and IHC (P) applications and shows reactivity with Human and Non-human primate samples.The MA5-13281 immunogen is human pheochromocytoma.靶标信息Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocine cells. CHGA is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides act as autocine or paracrine negative modulators of the neuroendocrine system. CHGA is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

数据

Chromogranin A Antibody (MA5-13281) in IHCImmunohistochemistry was performed on human pancreas tissue. Tissue was deparaffinized with xylene, followed by rehydration in sequential washes of 100% ethanol, 95% ethanol, 80% ethanol, 70% ethanol, and water. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and heated for 8-15 min. Following antigen retrieval, tissues were blocked in a 3% H2O2-methanol solution for 15 minutes at room temperature. Tissue was then probed with a Chromogranin A Mouse Monoclonal antibody (Product # MA5-13281) at a dilution of 1:100 in 3% BSA in PBS overnight at 4°C in a humidified chamber. Negative control tissue received no primary antibody. Tissues were washed extensively with PBST, and detection was performed using a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 followed by colorimetric detection using metal enhanced DAB. Tissues were then counterstained with hematoxylin and prepped for mounting and imaging

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.BMC cancerBevacizumab plus octreotide and metronomic capecitabine in patients with metastatic well-to-moderately differentiated neuroendocrine tumors: the XELBEVOCT study."MA5-13281 was used in immunohistochemistry to study the performance of the XELBEVOCT therapeutic regimen in patients with well-to-moderately differentiated neuroendocrine tumors"2.Human pathologyHuman papillomavirus-associated adenocarcinoma of the base of the tongue."MA5-13281 was used in immunohistochemistry to report on two cases of adenocarcinoma of the base of the tongue associated with HPV infection"3.The ProstateHuman ASH-1 promotes neuroendocrine differentiation in androgen deprivation conditions and interferes with androgen responsiveness in prostate cancer cells."MA5-13281 was used in immunohistochemistry to study the role of the human ASH-1 transcription factor in the development of the prostate cancer cell neuroendocrine phenotype"4.The American journal of surgical pathologyTumor staging but not grading is associated with adverse clinical outcome in neuroendocrine tumors of the appendix: a retrospective clinical pathologic analysis of 138 cases."MA5-13281 was used in immunohistochemistry to study the association of tumor staging with clinical outcome in neuroendocrine tumors of the appendix"5.Virchows Archiv : an international journal of pathologyHuman achaete-scute homolog-1 expression in neuroendocrine breast carcinoma."MA5-13281 was used in immunohistochemistry to study the expression of the hASH-1 transcription factor in neuroendocrine breast carcinoma"AuthorsRighi L,Rapa I,Votta A,Papotti M,Sapino A

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-44046.htm