CD44 Monoclonal Antibody (5F12)

货号：MA5-12394

规格：500 µL

价格：4733

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号： 5F12

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	10 µg/mL(ICC/IF)；Assay-dependent(Flow)

产品详细信息MA5-12394 has been successfully used in immunofluorescence analysis of CD44 in human bone marrow derived mesenchymal stem cells.靶标信息CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

数据

CD44 Antibody (MA5-12394) in ICC/IFImmunofluorescent analysis of CD44 (red) in human bone marrow derived mesenchymal stem cells. The cells were fixed with 2% paraformaldehyde, and blocked with 2% normal donkey serum diluted in PBS for 30 minutes at room temperature. Cells were stained with a CD44 monoclonal antibody (Product # MA5-12394) at a dilution of 10 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate (Product # A-21422) at a dilution of 1:800 for 1 hour at room temperature (red). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Note: Data courtesy of Innovators Program.

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参考文献：

1.Scientific reportsIGFBP-3 Blocks Hyaluronan-CD44 Signaling, Leading to Increased Acetylcholinesterase Levels in A549 Cell Media and Apoptosis in a p53-Dependent Manner."MA5-12394 was used in MTT reduction assay to show that IGFBP-3 or its peptide blocks hyaluronan-CD44 signaling via a mechanism that depends on both p53 and acetylcholinesterase."2.OncogeneSerglycin in tumor microenvironment promotes non-small cell lung cancer aggressiveness in a CD44-dependent manner."Published figure using CD44 monoclonal antibody (Product # MA5-12394) in Flow Cytometry"3.Journal of immunology (Baltimore, Md. : 1950)Hyaluronic acid or TNF-alpha plus fibronectin triggers granulocyte macrophage-colony-stimulating factor mRNA stabilization in eosinophils yet engages differential intracellular pathways and mRNA binding proteins."MA5-12394 was used in western blot to investigate the effect of tumor necrosis factor alpha and hyaluronic acid on the long-term eosinophil survival and its mechanism"4.Nature communicationsBi-directional cell-pericellular matrix interactions direct stem cell fate."MA5-12394 was used for blocking to show that human bone marrow stromal cells encapsulated within hyaluronic acid-based hydrogels modify their surroundings by synthesising, secreting and arranging proteins pericellularly or by degrading the hydrogel."5.OncogeneSerglycin in tumor microenvironment promotes non-small cell lung cancer aggressiveness in a CD44-dependent manner."Published figure using CD44 monoclonal antibody (Product # MA5-12394) in Flow Cytometry"

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