CD44 Monoclonal Antibody (156-3C11)

货号：MA5-13890

规格：500 µL

价格：4733

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG2a

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.2 mg/mL	用法：	1-2 µg/mL(WB)；1-2 µg/mL(IHC (P))；2 µg/mL(ICC/IF)；2 µg/test (Flow)；2 µg/mL(IP)

产品详细信息MA5-13890 targets CD44 in FACS, IHC (P), ICC/IF, IP and WB applications and shows reactivity with Human, Baboon and Non-human primate samples.The MA5-13890 immunogen is stimulated human leukocytes.MA5-13890 was used in the immunofluorescence analysis to successfully detect CD44 in human mesenchymal stem cells derived from iPSCs.靶标信息CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

数据

CD44 Antibody (MA5-13890) in ICC/IFImmunofluorescence analysis of CD44 Monoclonal Antibody was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD44 (156-3C11) Mouse Monoclonal Antibody (Product # MA5-13890) at 2 µg/mL in 0.1% BSA, incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

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参考文献：

1.The Journal of biological chemistryExtracellular Domains I and II of cell-surface glycoprotein CD44 mediate itstrans-homophilic dimerization and tumor cluster aggregation."MA5-13890 was used in Western Blotting to examine the biochemical properties of CD44 in homotypic tumor cell aggregation."2.Nature communicationsCDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity."MA5-13890 was used in Western Blotting to suggest targeting CDK5/TRIM59 signaling axis as a putative strategy for treating glioblastoma."3International journal of molecular sciencesRole of Corneal Stromal Cells on Epithelial Cell Function during Wound Healing."Published figure using CD44 monoclonal antibody (Product # MA5-13890) in Western Blot"4.CancersA Comprehensive Molecular and Clinical Analysis of the piRNA Pathway Genes in Ovarian Cancer."MA5-13890 was used in Immunohistochemistry (Paraffin) to show a piRNA pathway genes have key roles in the development of malignant neoplasms."5.CancersA Comprehensive Molecular and Clinical Analysis of the piRNA Pathway Genes in Ovarian Cancer."MA5-13890 was used in Immunohistochemistry (Paraffin) to show a piRNA pathway genes have key roles in the development of malignant neoplasms."

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