Human Hematopoietic Lineage Antibody Cocktail, eFluor 450, eBioscience™

货号：22-7775-72

规格：50 Tests

价格：6680

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG3

克隆号：RPA-2.10, OKT3, 61D3, CB16, HIB19, TULY56, HIR2

荧光染料：eFluor 450

类型:	单抗	同型对照：
浓度：	20 µL/Test	用法：	20 µL/test(Flow)

Description: The Human Hematopoietic Lineage eFluor® 450 Cocktail contains eFluor® 450-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.This cocktail contains the following antibodies:Anti-Human CD2 (RPA-2.10) eFluor® 450Anti-Human CD3 (OKT3) eFluor® 450Anti-Human CD14 (61D3) eFluor® 450Anti-Human CD16 (CB16) eFluor® 450Anti-Human CD19 (HIB19) eFluor® 450Anti-Human CD56 (TULY56) eFluor® 450Anti-Human CD235a (HIR2) eFluor® 450Applications Reported: Human Hematopoietic Lineage eFluor® 450 Cocktail has been reported for use in flow cytometric analysis.Applications Tested: Human Hematopoietic Lineage eFluor® 450 Cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.靶标信息Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.仅用于科研。不用于诊断过程。

数据

Human Hematopoietic Lineage Antibody (22-7775-72) in FlowNormal human peripheral blood cells were stained with Anti-Human CD34 FITC (Product # 11-0349-42), Anti-Human CD38 PE (Product # 12-0388-42), and the Human Hematopoietic Lineage eFluor® 450 Cocktail. Total viable cells were used for analysis. Cells in the right dot plot are gated on lineage negative events, depicted in the left histogram.

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参考文献：

1.The Journal of experimental medicineWASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner."22-7775 was used in Flow cytometry/Cell sorting to show that conditional WASH deletion in the haematopoietic system causes defective blood production of the host."AuthorsXia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

技术参数

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