Neurofilaments 70/200 kDa Monoclonal Antibody (2F11)

货号：MA5-15234

规格：500 µL

价格：4880

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG1

克隆号：2F11

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1:50-1:500(WB)；1:50-1:100 (IHC (P))；1:250(ICC/IF)；10 µg/mL(IP)

产品详细信息MA5-15234 targets Neurofilament (200kDa and 68kDa) in IF, IHC (P), IP, and WB applications and shows reactivity with Feline, Human, mouse, Rabbit, and Rat samples.The MA5-15234 immunogen is neurofilament purified from human brain.靶标信息Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity)

数据

Neurofilaments 70/200 kDa Antibody (MA5-15234) in ICC/IFImmunofluorescence analysis of CD79a was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD79a (2F11) Mouse Monoclonal Antibody (Product # MA5-15234) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification

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参考文献：

1.International urology and nephrologyProtective effect of Antrodia camphorata on bladder ischemia/reperfusion injury."MA5-15234 was used in immunohistochemistry to study the protective effect of Antrodia camphorata in bladder ischemia/reperfusion injury"2.Neurourology and urodynamicsCoenzyme Q10 diminishes ischemia-reperfusion induced apoptosis and nerve injury in rabbit urinary bladder."MA5-15234 was used in immunohistochemistry to study the role of Coenzyme Q in reducing ischemia-reperfusion-induced apoptosis and nerve damage in rabbit bladder"AuthorsJuan YS,Chuang SM,Mannikarottu A,Huang CH,Li S,Schuler C,Levin RM3.BJU internationalFree radical damage as a biomarker of bladder dysfunction after partial outlet obstruction and reversal."MA5-15234 was used in immunohistochemistry to study protein carbonylation and nitrotyrosination as a biomarker of dysfunction after partial bladder outlet obstruction."4.American journal of physiology. Regulatory, integrative and comparative physiologyEffects of L-arginine and L-NAME on chronic partial bladder outlet obstruction in rabbit."MA5-15234 was used in immunohistochemistry to investigate the impact of nitric oxide synthase and blook flow on the severity of damages caused by acute and chronic partial bladder outlet obstruction"5.Neuroscience lettersElectrophysiological measurements in three-dimensional in vivo-mimetic organotypic cell cultures: preliminary studies with hen embryo brain spheroids."MA5-15234 was used in immunohistochemistry to investigate the electrophysiological properties of three-dimensional hen embryo brain spheroids"

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