| 产品介绍 | | |
| Mouse anti Pig CD14, clone MIL2recognizes porcine CD14. Clone MIL2 was clustered as porcine CD14 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haversonet al.2001) . Mouse anti Pig CD14, clone MIL2 immunoprecipitates a protein of ~50 kDa consistent with the expected apparent molecular weight of porcine CD14, and demonstrates the expected CD14 profile by dual labelling and competition studies. Further, pre-incubation of peripheral blood monocytes with MIL2 inhibits the binding of FITC labelled LPS, consistent with masking the CD14 LPS binding site (Thackeret al.2001) .Mouse anti pig CD14, clone MIL2 demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry with a similar expression pattern to the anti human CD14 clone TüK4, lymphocytes and eosinophils are negative for MIL2 staining (Zelnickovaet al.2007). Cloning and characterization of porcine CD14 indicates a high degree of both functional and structural conservation when compared to CD14 from other mammalian species, the gene maps to chromosome 2 and is expressed on a wide range of tissues in a manner consistent with expression on myeloid cells. (Petersenet al.2007,Sanzet al.2007). |
| 产品详情 | | |
Target Species | Pig |
Product Form | Purified IgG conjugated to Alexa Fluor 647 - liquid |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Human | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
| Carrier Free | Yes |
Immunogen | Porcine peripheral blood lymphocytes. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
Fusion Partners | Spleen cells from immunized Balb/c mice were fused with cells from the P2-X63-Ag.653 mouse myeloma. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®647 | 650 | 665 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells in 100ul. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2b Negative Control:Alexa Fluor® 647 | MCA691A647 | F | 100 Tests |
| 数据 |
| Figure A. FITC conjugated Mouse anti Porcine CD14 antibody, clone MIL2 (MCA1218F) and A647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Porcine CD14 antibody, clone MIL2 (MCA1218F) and A647 conjugated Mouse anti Porcine CD45 antibody, clone K252.1E4 (MCA1222A647). All experiments performed on red cell lysed porcine blood gated on mononuclear cells. |
| 文献 |
| 1. Uitterdijk, A.et al.(2017) Time course of VCAM-1 expression in reperfused myocardial infarction in swine and its relation to retention of intracoronary administered bone marrow-derived mononuclear cells.PLoS One. 12 (6): e0178779.2. Sánchez, E.G.et al.(2017) Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production.Sci Rep. 7 (1): 10369.3. Fernández-Caballero, T.et al.(2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.Dev Comp Immunol. 81: 95-104.4. Sautter, C.A.et al.(2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.Dev Comp Immunol. 84: 181-92.5. López, E.et al.(2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.BMC Vet Res. 15 (1): 91.6. Forner, R.et al.(2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.PLoS One. 16 (5): e0249366.7. Skovdal, S.M.et al.(2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.PLoS One. 14 (10): e0223647.8. Vreman, S.et al.(2018) Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation.Dev Comp Immunol. 84: 361-70.9. Lau, C.et al.(2020) NHDL, a recombinant VL/VHhybrid antibody control for IgG2/4 antibodies.MAbs. 12 (1): 1686319.10. Nielsen, O.L.et al.(2021) A porcine model of subcutaneousStaphylococcus aureus.infection: a pilot study.APMIS. Mar 01 [Epub ahead of print]. |
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