Bio-Rad MCA1267SBV475 CD4抗体,Mouse anti Human CD4:StarBright Violet 475-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1267SBV475 CD4抗体,Mouse anti Human CD4:StarBright Violet 475

2024-10-23

Mouse anti Human CD4:StarBright Violet 475

货号：MCA1267SBV475

规格：100 Tests/0.5ml

价格：4276

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD4

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：RPA-T4

荧光染料：StarBright Violet 475

产品介绍
Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a 55 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.Mouse anti human CD4 antibody, clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (Piatier-Tonneau et al, 1997). Bio-Rad recommend the use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) for this purpose.
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to StarBright Violet 475 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 0.1% Pluronic F68 0.1% PEG 3350 0.05% Tween 20
Immunogen	Human PHA blasts
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
StarBright Violet 475	405	479
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
Acknowledgements	This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
存储条件
Store at +4°C. DO NOT FREEZE.This product should be stored undiluted.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 5ul of the suggested working dilution to label 10⁶cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
数据
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Violet 475 conjugated Mouse anti Human CD4 (MCA1267SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
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文献
1.monocytes.J Immunol. 167 (8): 4593-9.2.Voehringer, D.et al.(2002) Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1).Blood. 100 (10): 3698-702.3.Piatier-Tonneau, D. (1997) CD4 workshop panel report.In: Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998.4.Pentón-Rol, G.et al.(2011) C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.Int Immunopharmacol. 11 (1): 29-38.5.Wright, G.J.et al.(2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.Immunology. 102 (2): 173-9.6.Zhang, Y.et al.(2013) Acceleratedin vivo.proliferation of memory phenotype CD4+T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.PLoS Pathog. 9 (4): e1003310.7.Bughani, U.et al.(2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.PLoS One. 12 (7): e0180088.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1267SBV475 CD4抗体,Mouse anti Human CD4:StarBright Violet 475

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