Thermo CellBlox™ Blocking Buffer B001T03F01,CellBlox™ Blocking Buffer

2024-10-23

CellBlox™ Blocking Buffer

货号: B001T03F01,B001T02F01,B001T06F01

规格:100 tests,250 tests,500 tests

价格:1168,389,4670

产品类型:固定破膜剂

品牌:Thermo Fisher

物种:人/小鼠/非人灵长类/猪/恒河猴

宿主:驴

抗体亚型:IgG3

荧光染料:Alexa Fluor 488

The Invitrogen™ CellBlox™ Blocking Buffer is formulated to block nonspecific binding of Invitrogen™ NovaFluor™ labels with cells. These nonspecific interactions can result in higher background labeling. CellBlox Blocking Buffer is a non-antibody, non-protein–based blocking solution, and should be used every time a NovaFluor dye is used for labeling any cell type to minimize background labeling. (Figure 1)CellBlox Blocking Buffer is also recommended for use with cyanine-based dyes or cyanine-based tandem dyes to block non-specific interactions with monocytes and macrophages to minimize background labeling. (Figure 2)Use of CellBlox Blocking Buffer requires minimal change to most flow cytometry staining protocols. Add 5 µL CellBlox Blocking Buffer directly to a cell suspension containing 103- 108cells prior to the addition of an antibody, with 100 µL as a final staining volume. CellBlox Blocking Buffer may be added to an antibody cocktail mixture prior to labeling cells, by adding 5 µL CellBlox Blocking Buffer for every stained sample to be labeled with the antibody cocktail mixture, with 100 µL as a final staining volume.• Always use CellBlox Blocking Buffer with NovaFluor dyes when labeling cells for best background reduction.• CellBlox Blocking Buffer is compatible with all fluorophores and with Invitrogen™ LIVE/DEAD™ Fixable Dead Cell Stains.• CellBlox Blocking Buffer can be used with any fluorophore-antibody conjugate as a high-performance monocyte and macrophage blocking solution.• CellBlox Blocking Buffer is compatible with other blocking reagents, such as Fc Block, blocking proteins, Brilliant Stain Buffer, and Super Bright Complete Staining Buffer.• CellBlox Blocking Buffer is not required when labeling antibody-capture beads

优点:

-独特的光谱特征-同一荧光染料,不同荧光亮度-减少不同激光器交叉激发同一染料 • 以其激发光和发射光波长命名-在使用NovaFluor染料标记细胞时,使用 Invitrogen™ CellBlox™ 单核-巨噬细胞封闭缓冲液 (货号 B001T03F01)封闭非特异性标记,减少 背景噪音

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