Bio-Rad MCA1940SBUV400 人CD19抗体,Mouse anti Human CD19:StarBright UltraViolet 400

2024-10-23

Mouse anti Human CD19:StarBright UltraViolet 400

货号:MCA1940SBUV400

规格:100 Tests/0.5ml

价格:4531

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD19

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:LT19

荧光染料:StarBright UltraViolet 400

产品介绍
Mouse anti Human CD19 antibody, clone LT19recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rieet al.1989).CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21 (Bradburyet al.1992), CD9, CD81 and CD82 (Horváthet al.1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzuttoet al.1987).
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to StarBright UltraViolet 400 - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin0.1% Pluronic F680.1% PEG 33500.05% Tween 20

Immunogen

Normal human peripheral blood lymphocytes.

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)

StarBright UltraViolet 400

347394

Regulatory

For research purposes only

Guarantee

12 monthsfrom dateof despatch

AcknowledgementsThis product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
存储条件
Store at +4°C. DO NOT FREEZE.This product should be stored undiluted.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry
Use 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
数据

Figure A. StarBright Violet 670 conjugated Mouse anti Human CD20 (MCA1710SBV670). Figure B. StarBright Violet 670 conjugated Mouse anti Human CD20 (MCA1710SBV670) and StarBright UltraViolet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
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文献
1. Girbl, T.et al.(2013) CD40-mediated activation of chronic lymphocytic leukemia cells promotes their CD44-dependent adhesion to hyaluronan and restricts CCL21-induced motility.Cancer Res. 73: 561-70.2. Hertzberg, L.et al.(2010) Down syndrome acute lymphoblastic leukemia, a highly heterogeneous disease in which aberrant expression of CRLF2 is associated with mutated JAK2: a report from the International BFM Study Group.Blood. 115: 1006-17.3. Kakko, T.et al.(2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.Diab Vasc Dis Res. 8: 221-8.4. Dorvignit, D.et al.(2012) Expression and biological characterization of an anti-CD20 biosimilar candidate antibody: a case study.MAbs. 4 (4): 488-96.5. Karlsen, M.et al.(2015) TLR-7 and -9 Stimulation of Peripheral Blood B Cells Indicate Altered TLR Signalling in Primary Sjögren's Syndrome Patients by Increased Secretion of Cytokines.Scand J Immunol. 82 (6): 523-31.6. Clark, L.E.et al.(2018) Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses.Nat Commun. 9 (1): 1884.7. Gu, Y.et al.(2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.Nat Commun. 10 (1): 893.8. Yang, C.et al.(2013) B cells promote tumor progression via STAT3 regulated-angiogenesis.PLoS One. 8 (5): e64159.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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