Bio-Rad MCA2127SBV710,Mouse anti Human CD25:StarBright Violet 710-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA2127SBV710,Mouse anti Human CD25:StarBright Violet 710

2024-10-23

Mouse anti Human CD25:StarBright Violet 710

货号：MCA2127SBV710

规格：100 Tests/0.5ml

价格：5466

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD25

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：MEM-181

荧光染料：StarBright Violet 710

产品介绍
Mouse anti Human CD25, clone MEM-181recognizes the ~55 kDa alpha subunit of the human IL-2 receptor, also known as p55 or TAC antigen, CD25 is a type 1 transmembrane protein withtwo Sushi domains, also known as short concensus repeats (SCRs) or complement control protein (CCP) modules (Normanet al. 1991) located within its extracellular domain.The IL-2 receptor exists in three forms. A high affinity form consisting of a non-covalently linked heterodimer composed of the alpha subunit (CD25) and the IL-2 receptor beta subunit also known as CD122 or p75, a medium affinity beta subunit (CD122) monomer or a low affinity alpha (CD25) subunit monomer.CD25 is expressed by activated T lymphocytes and activated B lymphocytes responding to antigen or mitogen stimulation. CD25 is also expressed in some thymocytes and oligodendrocytes. In disease, elevated expression of CD25 in noted in a number of chronic inflammatory conditions, tuberculoid leprosy patients demonstrate markedly elevated levels of circulating CD25high FoxP3+ regulatory T cells (T-regs) (Attiaet al.2010).Elevated levels of CD25 antigen expression are often seen in cases ofnon-Hodgkin 's lymphomaand diffuse large B cell lymphoma (Fujiwaraet al.2013).
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to StarBright Violet 710 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin0.1% Pluronic F680.1% PEG 33500.05% Tween 20
Immunogen	Human PHA blasts; day 3 of culture.
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
StarBright Violet 710	401	713
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Store at +4°C. DO NOT FREEZE.This product should be stored undiluted.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
数据
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 710 conjugated Mouse anti Human CD25 (MCA2127SBV710). All experiments performed on human PBMCs stimulated with PHA for 3 days gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
相关产品
Description	Product Code	Applications	Pack Size
Human Seroblock	BUF070A	F	50 Test
Human Seroblock	BUF070B	F	200 Test
文献
1. Zhang, Y.et al.(2013) Acceleratedin vivoproliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.PLoS Pathog. 9 (4): e1003310.2. Nocentini, G.et al.(2014) Expansion of regulatory GITR + CD25 Low/- CD4 + T cells in systemic lupus erythematosus patients.Arthritis Res Ther. 16: 444.3. Soukup, K.et al.(2015) The MAPK-Activated Kinase MK2 Attenuates Dendritic Cell-Mediated Th1 Differentiation and Autoimmune Encephalomyelitis.J Immunol. 195 (2): 541-52.4. Kusunoki, Y.et al.(2010) T-cell immunosenescence and inflammatory response in atomic bomb survivors.Radiat Res. 174 (6): 870-6.5. Bughani, U.et al.(2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.PLoS One. 12 (7): e0180088.6. Knutson, K.L.et al.(2015) Regulatory T cells, inherited variation, and clinical outcome in epithelial ovarian cancer.Cancer Immunol Immunother. 64 (12): 1495-504.7. Boland, J.W.et al.(2014) A preliminary evaluation of the effects of opioids on innate and adaptive humanin vitroimmune function.BMJ Support Palliat Care. 4 (4): 357-67.8. Luger, R.et al.(2013) Toll-like receptor 4 engagement drives differentiation of human and murine dendritic cells from a pro- into an anti-inflammatory mode.PLoS One. 8 (2): e54879.9. Rezalotfi, A.et al.(2020) Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells.J Immunol Res. 2020: 6261814.10. Thymianou, Set al. (2019) MBP7285 on Human Tcell ActivationMobile health Knoledge 21 Jul

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-44206.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

Bio-Rad MCA2127SBV710,Mouse anti Human CD25:StarBright Violet 710

相关推荐