人多能干细胞(PBMC来源)
货号:907005-PM342,907005-PM849,907005-PM853,907005-PM3,907005-PM726,907005-PM17,907005-PM2,907005-PM696,907005-PM705
规格:5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells,5×10e5 cells
价格:6000,6000,6000,6000,6000,6000,6000,6000,6000
产品类型:原代细胞
品牌:PBM
Human iPSCs 是由外周血细胞通过非整合型质粒电转的方法诱导获得。Human iPSCs 在多潜能干细胞培养体系中可以长期稳定快速增殖,具有高度近似人ES细胞的克隆形态和基因表达,长期维持正常核型,并在体内外具有三胚层分化潜能。 |
产品数据 |
| A:细胞形态观察. B: iPSC 表达多能性因子Sox2, OCT4, Nanog和 TRA-1-60,比例尺: 100μm。C: ICC检测结果。D: 在 NOD/SCID 小鼠中 iPSC 形成畸胎瘤。图为三个胚层(中胚层:骨;内胚层:腺体;外胚层:神经管)HE染色结果,比例尺:40μm。 |
注意:人胚胎干细胞(hES)或诱导多能干细胞(hiPS)在无滋养层细胞培养条件下培养换液时,请将新鲜的培养液沿培养容器壁缓慢添加,避免液体的冲击力将贴壁的细胞吹起脱离培养皿。 |
其它人iPSC |
货号 | 来源 | 建立方法 | HLA配型** |
907005-PM342 | PBMCs | Episomal | A*11:01:01 |
907005-PM849 | PBMCs | Episomal | A*24:02:01 |
907005-PM853 | PBMCs | Episomal | A*02:01:01 |
907005-PM3 | PBMCs | Episomal | A*11:01:01 |
907005-PM726 | PBMCs | Episomal * | A*11:01:01 |
907005-PM17 | PBMCs | Episomal | A*11:01:01 |
907005-PM2 | PBMCs | Episomal | A*03:02:01G |
907005-PM696 | PBMCs | Episomal * | A*11:02:01G |
907005-PM705 | PBMCs | Episomal | A*24:02:01 |
907005-T849 | T cell | Sendai virus | A*24:02:01 |
907005-T853 | T cell | Sendai virus | A*02:01:01 |
907005-T17 | T cell | Sendai virus | A*11:01:01 |
907005-CBP35 | CBMNC | Episomal | A*01:01:01 |
907005-CB52 | CB CD34 | Episomal | A*02:01:01 |
相关产品 |
·SepTube-50密度梯度离心管(#601015)·人外周血淋巴细胞分离液(#LTS1077)·MEF细胞(#903510)·Mitomycin C丝裂霉素(#216010)·Episomal iPSC Reprogramming Kit/IPS重编程试剂盒(#611005)·HapClut™ 0.1% Gelatin (#211050)·HapClult™ Erythroid cell expansion medium(用于分离的 PBMCs 细胞培养)(#631005)·HapClut™ iPS reprogramming medium (#621050)·低氧培养盒(#504001)·Single Flow Meter for Hypoxia Chamber流量计(#504002)·HapClut™ iPS Culture medium(#622050) |
参考文献 |
1. Wei Wen#, Jian-Ping Zhang#, Jing Xu#, Ruijun Jeanna Su, Amanda Neises, Guang-Zhen Ji, Weiping Yuan, Tao Cheng*, Xiao-Bing Zhang* (2016). Enhanced Generation of Integration- free iPSCs from Human Adult Peripheral Blood Mononuclear Cells with an Optimal Combination of Episomal Vectors. Stem Cell Reports, 6(6):873-884.2. Wei Wen,Jian-Ping Zhang,Wanqiu Chen,Cameron Arakaki,Xiaolan Li, David Baylink, Gary D. Botimer,Jing Xu,Weiping Yuan,Tao Cheng*, Xiao-Bing Zhang* (2017). Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood MononuclearCells Using Episomal Vectors. J. Vis. Exp. (119), e55091, doi:10.3791/55091.3. Wei Wen, Xinxin Cheng, Yawen Fu, Feiying Meng, Jian-Ping Zhang, Lu Zhang, Xiao-Lan Li, Zhixue Yang, Jing Xu, Feng Zhang, Gary D. Botimer, Weiping Yuan, Changkai Sun, Tao Cheng*, and Xiao-Bing Zhang* (2018). High-Level Precise Knockin of iPSCs by Simultaneous Reprogramming and Genome Editing of Human Peripheral Blood Mononuclear Cells, Stem Cell Reports, https://doi.org/10.1016/j.stemcr.2018.04.013 |
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