产品介绍

Reagents in the Kit

2 vials ReadiLink Labeling Dye (powder) (Note:Use one vial to label 50 μg of antibodies)1 vial (20 μl) Reaction Buffer1 vial (20 μl) Quench Buffer.

Regulatory

For research purposes only

Guarantee

Guaranteed until date of expiry. Please see product label.

存储条件

应用

Verified

Min Dilution

Max Dilution

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Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

• Instructions For Use

• Important:Thaw all kit components prior to use.Note:The antibody of interest must be suspended in phosphate buffered saline (PBS), pH 7.2-7.4, or be dialyzed against PBS prior to conjugation to remove free amines or ammonium salts in the solution.Note:The optimal antibody conjugation is 1 mg/ml. A conjugation performed at a different conjugation concentration may cause suboptimal labeling.1. Suspend the antibody of interest in PBS to create a 1 mg/ml concentration.2. Add 5 μl reaction buffer to 50 μl antibody solution from step 1.3. Mix well by pipetting up and down a few times.4. Add the entire volume (55 μl) toonevial of labeling dye and mix by pipetting.5. Incubate for 60 min at room temperature.6. Add 5 μl quencher to the reaction mixture.7. Incubate for 10 min at room temperature.Antibodies are now labeled and ready to use.

Peripheral blood lymphocytes stained with Mouse anti human CD3 antibody, clone UCHT1 (MCA463G) conjugated with the ReadiLink 555/570 Antibody Labeling Kit (1351003) to detect T cells. Data acquired on the ZE5 Cell Analyzer.