CD79a Monoclonal Antibody (24C2.5), FITC

货号：11-0791-80

规格：25μg

价格：925

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG

克隆号：24C2.5

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度：	0.5 mg/mL	用法：	1 μg/test(Flow)

产品详细信息Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: FITC Mouse IgG1 K Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: This Mouse IgG1 K Isotype Control can be used at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1.PloS oneGuanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types."11-4714 was used in Flow cytometry/Cell sorting to statistically analyse miRNA microarray data, revealing that guanine-rich sequences are a dominant feature of exosome-dominant miRNAs."AuthorsMomose F,Seo N,Akahori Y,Sawada S,Harada N,Ogura T,Akiyoshi K,Shiku H2.Molecular cancerHOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells."11-4714 was used in Flow cytometry/Cell sorting to to study the role of HOXC8 regulation in breast cancer stem cells."AuthorsShah M,Cardenas R,Wang B,Persson J,Mongan NP,Grabowska A,Allegrucci C3.Stem cell reportsMIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia."11-4714 was used in Flow cytometry/Cell sorting to identify the hypoxia induced action of MIF as a transcriptional regulator of SOX9 and RUNX2."AuthorsYao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y4.International journal of clinical and experimental pathologyPDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo."11-4714 was used in Flow cytometry/Cell sorting to study the characteristics and differentiation potential of PDGFR-β (+) perivascular cells from infantile hemangioma."AuthorsYuan SM,Guo Y,Zhou XJ,Shen WM,Chen HN5.Journal of immunological methodsIsolation of biologically-active exosomes from human plasma."11-4714 was used in Flow cytometry/Cell sorting to isolate and study the effects of exosomes present in human plasma on immune cells."AuthorsMuller L,Hong CS,Stolz DB,Watkins SC,Whiteside TL

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