IL-8 Human ELISA Kit EIISA试剂盒,IL-8 Human ELISA Kit/人IL-8 EIISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-8 Human ELISA Kit EIISA试剂盒,IL-8 Human ELISA Kit/人IL-8 EIISA试剂盒

2024-10-23

IL-8 Human ELISA Kit/人IL-8 EIISA试剂盒

货号：KAC1301

规格：96 tests

价格：7030

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.7 pg/mL
检测范围	7-750 pg/mL
样本类型/体积	上清液 100 μL 血清 100μL 血浆 100μL
操作时间	1 hr 20 min
测试时间	2 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	3.7%
批内变异系数（CV）	4.2%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 标准稀释液 - 孵育缓冲液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 对照
储存温度	2-8℃
Protein name	IL-8
Protein aliases	(Ala-IL-8)77, (Ser-IL-8)72, alveolar macrophage chemotactic factor I, beta endothelial cell-derived neutrophil activating peptide, beta-thromboglobulin-like protein, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, GCP-1, GCP/IL-8 protein I, GCP/IL-8 protein II, GCP/IL-8 protein III, GCP/IL-8 protein IV, GCP/IL-8 protein V, GCP/IL-8 protein VI, Granulocyte chemotactic protein 1, IL-8, IL-8(1-77), IL-8(5-77), IL-8(6-77), IL-8(7-77), IL-8(8-77), IL-8(9-77), IL8/NAP1 form I, IL8/NAP1 form II, IL8/NAP1 form III, IL8/NAP1 form IV, IL8/NAP1 form V, IL8/NAP1 form VI, interleukin 8, Interleukin-8, lung giant cell carcinoma-derived chemotactic protein, lymphocyte derived neutrophil activating peptide, Lymphocyte-derived neutrophil-activating factor, LYNAP, MDNCF, MDNCF-a, MDNCF-b, MDNCF-c, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAF, NAP-1, Neutrophil-activating factor, neutrophil-activating peptide 1, Neutrophil-activating protein 1, Protein 3-10C, small inducible cytokine subfamily B, member 8, T-cell chemotactic factor, tumor necrosis factor-induced gene 1
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	CXCL8, GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1
Gene ID	(Human) 3576
Gene symbol	CXCL8
UniProt ID	(Human) P10145

The Human Interleukin 8 (Hu IL-8) ELISA quantitates Hu IL-8 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-8.人白介素8（人IL-8）ELISA可以定量人血清，血浆，缓冲液或细胞培养基中的人IL-8。该检测试剂盒将专门识别天然和重组的人IL-8。Principle of the method 方法原理The HumanIL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-8固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.白介素8（IL-8，CXCL8）是一种72个氨基酸的促炎因子，属于趋化因子的CXC亚家族，并与细胞表面受体IL-8RA和IL-8RB结合。 IL-8用作化学吸引剂和有效的血管生成因子。 IL-8的表达和分泌可通过多种细胞（包括巨噬细胞和内皮细胞）中的多种炎症刺激来诱导。在内皮细胞中，IL-8存在于称为Weibel-Palade体的储存小泡中。首先从骨肉瘤细胞中分离出来的IL-8含有ELR-基序（N端Glu-Leu-Arg氨基酸序列），并通过CXCR1和CXCR2受体发出信号。 IL-8的先前命名法包括嗜中性粒细胞激活蛋白1（NAP-1），粒细胞趋化蛋白1（GCP-1），单核细胞衍生的嗜中性粒细胞激活肽（MONAP）和蛋白3-10C。 IL-8和CXC趋化因子基因家族的其他十个成员在映射到染色体4q的区域中形成趋化因子基因簇。癌症研究表明IL-8在肿瘤的血管生成和生长中起作用，IL-8在毛细支气管炎（一种由病毒感染引起的常见呼吸道疾病）的发病机理中起作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-8 Human ELISA KitRepresentative standard curve for Human IL-8 ELISA

技术参数

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IL-8 Human ELISA Kit EIISA试剂盒,IL-8 Human ELISA Kit/人IL-8 EIISA试剂盒

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