HGH Human ELISA Kit ELISA试剂盒,HGH Human ELISA Kit/人HGH ELISA试剂盒

2024-10-23

HGH Human ELISA Kit/人HGH ELISA试剂盒

货号:KAQ1081

规格:96 tests

价格:7031

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.17 µIU/mL

检测范围0.45-98 µIU/mL

样本类型/体积

血清 50μL

血浆 50μL

操作时间

1 hr 20 min

测试时间

1 hr

Homogenous (no wash)

No

批间变异系数(CV)6.6%
批内变异系数(CV)5.2%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 标准稀释液

- 孵育缓冲液

- HRP-共轭检测抗体

- 洗涤液

- 显色剂

- 终止液

- 对照

储存温度

2-8℃

Protein nameGrowth Hormone
Protein aliasesGH, Growth hormone, Growth hormone 1, growth hormone B5, GH-N, Pituitary growth hormone, Somatotropin

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物HRP
Gene aliasesGH, GH-N, GH1, GHB5, GHN, hGH-N, IGHD1B
Gene ID(Human) 2688
Gene symbolGH1
UniProt ID

(Human) P01241

The Human Growth Hormone (Hu GH) ELISA quantitates Hu GH in human serum and plasma. The assay will exclusively recognize both natural and recombinant Hu GH.人生长激素(人GH)ELISA可以定量人类血清和血浆中的人GH。 该检测试剂盒将只识别天然和重组人GH。Principle of the method 方法原理The Human GH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人GH固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Human growth hormone (hGH) is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The GH1 gene, along with 4 additional related genes, is located at the growth hormone locus on chromosome 17. The five genes share a remarkably high degree of sequence identity. Alternative splicing results in 5 isoforms ranging from approximately 19 kDa to 24 kDa. HGH is produced by the somatotroph cells in the pituitary. It acts through binding to membrane receptors that belong to the cytokine receptor subfamily. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Mutations or deletions in the gene lead to growth hormone deficiency and short stature.人生长激素(hGH)是生长激素/催乳激素家族的成员,在生长控制中起着重要作用。 GH1基因和4个其他相关基因一起位于17号染色体上的生长激素基因座。这五个基因具有非常高的序列同一性。 选择性剪接导致5个同工型,范围从大约19 kDa到24 kDa。 HGH由垂体中的生长体细胞产生。 它通过与属于细胞因子受体亚家族的膜受体结合而起作用。 它在刺激人体生长中的主要作用是刺激肝脏和其他组织分泌IGF-1。 它刺激成肌细胞的分化和增殖。 它还刺激肌肉和其他组织中的氨基酸吸收和蛋白质合成。 基因的突变或缺失会导致生长激素缺乏和身材矮小。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

HGH Human ELISA KitThe following data are for demonstration purposes only and cannot be used in place of data generated at the time of assay.

技术参数
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