Cytokeratin 5 Monoclonal Antibody (SP27)/KRT5单抗

货号：MA5-16372

规格：500 µL

价格：6288

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：Synthetic peptide from C-terminus of human cytoker

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

克隆号：SP27

荧光染料：其它

类型:	单抗	同型对照：	IgG
浓度：		用法：	1:100(Flow);1:100(ICC);1:100(IF);1:30(IHC(F));1:100(IHC(P));1:100(WB)

产品详细信息Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, breast, prostate, prostate carcinoma or mesothelioma.靶标信息Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

Cytokeratin 5 Antibody (MA5-16372) in WBWestern blot analysis of A431 Cells using anti-Keratin 5 Monoclonal Antibody (Product # MA5-16372). The recommened dilution for this antibody in western blot applications is 1:100. Cytokeratin 5 Antibody (MA5-16372) in IHCImmunohistochemical analysis of Keratin 5 using anti-Keratin 5 Monoclonal Antibody (Product # MA5-16372) in Prostate Adenocarcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.

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参考文献：

1.HistopathologyImmunohistochemistry in the diagnosis of papillary lesions of the breast.2.EndocrinologyB-cell maturation antigen (BCMA) activation exerts specific proinflammatory effects in normal human keratinocytes and is preferentially expressed in inflammatory skin pathologies.

技术参数

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