Granzyme B Human ELISA Kit ELISA试剂盒,Granzyme B Human ELISA Kit/人颗粒酶B ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Granzyme B Human ELISA Kit ELISA试剂盒,Granzyme B Human ELISA Kit/人颗粒酶B ELISA试剂盒

2024-10-23

Granzyme B Human ELISA Kit/人颗粒酶B ELISA试剂盒

货号：BMS2027,BMS2027TEN

规格：96 Tests,10X96 Tests

价格：0,0

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.2 pg/mL
检测范围	0.66-480 pg/mL
样本类型/体积	血清 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	2 hr 45 min
Homogenous (no wash)	No
批间变异系数（CV）	10.4%
批内变异系数（CV）	8.5%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	Granzyme B
Protein aliases	C11, Cathepsin G-like 1, CTLA-1, CTSGL1, cytotoxic serine protease B, Cytotoxic T-lymphocyte proteinase 2, cytotoxic T-lymphocyte-associated serine esterase 1, fragmentin 2, Fragmentin-2, granzyme 2, Granzyme B, granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1), Granzyme-2, HLP, Human lymphocyte protein, Lymphocyte protease, SECT, T-cell serine protease 1-3E
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, GRB, GZMB, HLP, SECT
Gene ID	(Human) 3002
Gene symbol	GZMB
UniProt ID	(Human) P10144

The Human Granzyme B ELISA quantitates Hu Granzyme B in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Granzyme B.人GranzymeB ELISA可以定量人血清或细胞培养基中GranzymeB。该检测试剂盒将专门识别天然和重组人GranzymeB。Principle of the method 方法原理The Human Granzyme B solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人Granzyme B固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.颗粒酶B是颗粒酶丝氨酸蛋白酶家族的成员，存在于细胞毒性T细胞和NK细胞的颗粒中。颗粒酶B已被描述为CGL1（组织蛋白酶G样-1），一种仅在细胞活化后在细胞毒性T淋巴细胞中表达的丝氨酸蛋白酶，而CTLA-1（与细胞毒性T淋巴细胞相关的丝氨酸酯酶1）则基于mRNA的鉴定。各种细胞毒性T细胞，但未在非细胞毒性淋巴样细胞中观察到。颗粒酶B对于通过凋亡快速诱导靶细胞死亡至关重要，凋亡是通过与细胞毒性T细胞相互作用诱导的。已经鉴定出参与该过程的受体是6-磷酸甘露糖受体，其在细胞毒性T细胞诱导的细胞凋亡期间充当粒酶B的死亡受体。颗粒酶B进入靶细胞以裂解caspase-3，并启动caspase级联反应，导致DNA片段化和凋亡。颗粒酶B还可以通过切割Bid蛋白来通过线粒体凋亡途径起作用。颗粒酶是中性的丝氨酸蛋白酶，它们存储在细胞毒性T淋巴细胞（CTL）的专门裂解颗粒中以及自然杀伤（NK）细胞中。已经从小鼠CTL和NK细胞中分离并克隆了许多颗粒酶（A至G），但是在人类中，已经克隆和鉴定的颗粒酶很少。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

Granzyme B Human ELISA KitRepresentative standard curve for Human Granzyme B ELISA

技术参数

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Granzyme B Human ELISA Kit ELISA试剂盒,Granzyme B Human ELISA Kit/人颗粒酶B ELISA试剂盒

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