CD44var (v6) (Soluble) Human ELISA Kit ELISA试剂盒,CD44var (v6) (Soluble) Human ELISA Kit/人CD44var (v6) (可溶)ELISA试剂盒

2024-10-23

CD44var (v6) (Soluble) Human ELISA Kit/人CD44var (v6) (可溶)ELISA试剂盒

货号:BMS210

规格:96 tests

价格:6120

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.05 ng/mL

检测范围0.32-10.0 ng/mL

样本类型/体积

血清 10 μL

血浆 10 μL

尿液 10 μL

上清液 20 μL

操作时间

1 hr 20 min

测试时间

3 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)4.2%
批内变异系数(CV)3%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- HRP-共轭检测抗体

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameCD44v6
Protein aliasesCD44, CD44 antigen, CDw44, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, hematopoietic cell E- and L-selectin ligand, Heparan sulfate proteoglycan, Hermes antigen, homing function and Indian blood group system, HUTCH-I, Hyaluronate receptor, PGP-1, PGP-I, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, soluble CD44

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物HRP
Gene aliasesCD44, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1
Gene ID(Human) 960
Gene symbolCD44
UniProt ID(Human) Q9UCB0
The Human CD44var (v6) ELISA quantitates Hu CD44var (v6) in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD44var (v6).人CD44var(v6)ELISA定量了人血清,血浆,尿液或细胞培养基中的人CD44var(v6)。 该检测试剂盒将专门识别天然和重组人CD44var(v6)。Principle of the method 方法原理The Human CD44var (v6) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人CD44var(v6)固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.CD44细胞表面抗原是一种100kDa糖蛋白,广泛表达于人白细胞,脑白质以及肠和乳房的某些上皮细胞。 存在几种CD44亚型,包括在许多正常组织中检测到的主要CD44H亚型。 CD44是透明质酸(HA)的受体,参与细胞间相互作用,细胞粘附和迁移。 CD44还参与多种细胞功能,包括淋巴细胞活化,再循环和归巢。 CD44的表达可能在某些癌中上调,并且推测这可能与转移潜能有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

CD44var (v6) (Soluble) Human ELISA KitRepresentative standard curve for Human CD44var v6 ELISA

技术参数
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