VEGF Receptor 2/KDR Human ELISA Kit ELISA试剂盒,VEGF Receptor 2/KDR Human ELISA Kit/人VEGF受体2 / KDR ELISA试剂盒

2024-10-23

VEGF Receptor 2/KDR Human ELISA Kit/人VEGF受体2 / KDR ELISA试剂盒

货号:BMS2019,BMS2019TEN

规格:96 Tests,10X96 Tests

价格:6139,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

7.0 pg/mL

检测范围78-5,000 pg/mL

样本类型/体积

血清 20 μL

血浆 20 μL

上清液 20 μL

操作时间

1 hr 20 min

测试时间

3 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)9.8%
批内变异系数(CV)5.3%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameFLK1 (VEGF Receptor 2)
Protein aliasesCD309, Fetal liver kinase 1, fetal liver kinase-1, FLK-1, KDR, Kinase insert domain receptor, kinase insert domain receptor (a type III receptor tyrosine kinase), Protein-tyrosine kinase receptor flk-1, soluble VEGFR2, tyrosine kinase growth factor receptor, Vascular endothelial growth factor receptor 2, VEGFR-2

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCD309, FLK1, KDR, VEGFR, VEGFR2
Gene ID(Human) 3791
Gene symbolKDR
UniProt ID(Human) P35968
The Human Vascular Endothelial Growth Factor Receptor 2/KDR (Hu VEGFR2) ELISA quantitates Hu VEGFR2 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VEGFR2.人血管内皮生长因子受体2 / KDR(人VEGFR2)ELISA定量了人血清,血浆,缓冲液或细胞培养基中的人VEGFR2。 该检测试剂盒将专门识别天然和重组人VEGFR2。Principle of the method 方法原理The Human VEGFR2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人VEGFR2固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of this gene are implicated in infantile capillary hemangiomas.血管内皮生长因子(VEGF)是内皮细胞的主要生长因子。 该基因编码VEGF的两个受体之一。 该受体称为激酶插入结构域受体,是III型受体酪氨酸激酶。 它是VEGF诱导的内皮细胞增殖,存活,迁移,肾小管形态发生和发芽的主要介质。 该受体的信号传导和运输受多种因素调节,包括Rab GTPase,P2Y嘌呤核苷酸受体,整联蛋白alphaVbeta3,T细胞蛋白酪氨酸磷酸酶等。该基因的突变与婴儿毛细血管血管瘤有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

VEGF Receptor 2/KDR Human ELISA KitRepresentative standard curve for Human VEGF Receptor 2 ELISA

技术参数
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