ICAM-1 (Soluble) Monkey ELISA Kit ELISA试剂盒,ICAM-1 (Soluble) Monkey ELISA Kit/猴ICAM-1(可溶)ELISA试剂盒

2024-10-23

ICAM-1 (Soluble) Monkey ELISA Kit/猴ICAM-1(可溶)ELISA试剂盒

货号:BMS648,BMS648TEN

规格:96 Tests,10X96 Tests

价格:6120,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

1.1 U/mL

检测范围6.3-100 U/mL

样本类型/体积

血清 10 μL

上清液 10 μL

操作时间

1 hr 20 min

测试时间

1 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)10%
批内变异系数(CV)5%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- HRP-共轭检测抗体

- 洗涤液

- 显色剂

- 终止液

- 对照

- 胶板盖

储存温度

2-8℃

Protein nameICAM-1 (CD54)
Protein aliasesCD54, ICAM-1, Intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor

物种(Tested)

狒狒,恒河猴

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物HRP
Gene aliasesICAM1
Gene ID(Rhesus monkey) 712280
Gene symbolICAM1
UniProt ID(Rhesus monkey) Q5NKV6
The Monkey Soluble Intercellular Adhesion Molecule-1 (ICAM-1) ELISA quantitates Mk ICAM-1 in monkey serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Mk ICAM-1.猴可溶性细胞间粘附分子1(ICAM-1)ELISA定量了猴血清或细胞培养基中的猴ICAM-1。 该检测试剂盒将专门识别天然和重组猴ICAM-1。Principle of the method 方法原理The Monkey ICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.猴 ICAM-1固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.ICAM-1(CD54)是一种85-110 kDa单链1型整合膜糖蛋白,具有五个免疫球蛋白超家族重复序列,一个跨膜区和一个胞质区的胞外域。 ICAM-1具有7个潜在的N-连接的糖基化位点,并且与ICAM-3(CD50)和ICAM-2(CD102)具有相当的氨基酸序列同源性。 ICAM-1与CD11a / CD18(白细胞粘附分子,LFA-1)或CD11b / CD18(Mac-1)类型的整合素结合,并被鼻病毒用作受体。 ICAM-1由活化的内皮细胞表达,并在上皮细胞,成纤维细胞,软骨细胞,B淋巴细胞,T淋巴细胞(低),单核细胞,巨噬细胞,树突状细胞和嗜中性粒细胞中检测到,其水平随着炎症而增加。 在某些癌细胞和黑色素瘤细胞中也检测到ICAM-1。 在血浆中可检测到可溶性ICAM-1,在患有各种炎症综合症的患者中可溶性ICAM-1升高。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

ICAM-1 (Soluble) Monkey ELISA KitRepresentative standard curve for monkey ICAM-1 ELISA

技术参数
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