IL-28A Mouse ELISA Kit ELISA试剂盒,IL-28A Mouse ELISA Kit/小鼠IL-28A ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-28A Mouse ELISA Kit ELISA试剂盒,IL-28A Mouse ELISA Kit/小鼠IL-28A ELISA试剂盒

2024-10-23

IL-28A Mouse ELISA Kit/小鼠IL-28A ELISA试剂盒

货号：BMS6028,BMS6028TEN

规格：96 Tests,10X96 Tests

价格：6120,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	12.85 pg/mL
检测范围	31.3-2,000 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	7.2%
批内变异系数（CV）	5.6%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-28A
Protein aliases	IFN-lambda-2, Interleukin-28A, IL-28A, IFN-lambda2, Interferon lambda-2, interferon-lambda2, interleukin 28A
物种（Tested）	小鼠
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	EG330496, Ifnl2, IL-28A, Il28a
Gene ID	(Mouse) 330496
Gene symbol	Ifnl2
UniProt ID	(Mouse) Q4VK74

The Mouse Interleukin-28A (Ms IL-28A) ELISA quantitates Ms IL-28A in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-28A.小鼠白介素28A（小鼠IL-28A）ELISA定量检测小鼠血清，血浆，缓冲液或细胞培养基中的小鼠IL-28A。该检测试剂盒将专门识别天然和重组小鼠IL-28A。Principle of the method 方法原理The Mouse IL-28A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠IL-28A固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons.The IFN lambdas signal through a heterodimeric receptor of which one subunit, IL-10R2, is shared with other members of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas. Signaling occurs through the Jak/STAT pathway in a similar manner as the type I IFN (IFN alpha/beta) and activates many of the same genes despite low sequence homology between the cytokines and receptors in the two families. Both IFN families display antiviral activity through the induction of antiviral protein production in target cells and the upregulation of MHC class I expression. These proteins also exhibit antiproliferative and antitumor effects, making them a possible alternative to IFN alpha cancer therapies. Unlike the type I IFN, which are able to stimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic and some tumor cells due to the limited expression of IFN lambda R1. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards the production of CD4+CD25+FoxP3+ regulatory T cells, suggesting a possible immunoregulatory role.IL-28A属于IFN λ家族，是IL-10超家族中的一种新型细胞因子家族。该家族的三个成员是IL-29（IFN λ 1），IL-28A（IFN λ 2）和IL-28B（IFN λ 3），也被称为III型干扰素。异源二聚体受体，其一个亚基IL-10R2与超家族的其他成员共享。第二个亚基是IFNλR1或IL-28Rα，是IFN λ所特有的。尽管两个家族中细胞因子和受体之间的序列同源性很低，但通过Jak / STAT途径发生信号的方式与I型IFN（IFNα/β）类似，并激活了许多相同的基因。两种IFN家族均通过诱导靶细胞中抗病毒蛋白的产生以及MHC I类表达的上调而显示出抗病毒活性。这些蛋白质还表现出抗增殖和抗肿瘤作用，使其成为IFNα癌症疗法的可能替代品。与能够刺激大多数细胞的I型IFN不同，由于IFNλR1的表达有限，对IFNλ刺激的反应似乎仅限于树突状细胞和某些肿瘤细胞。另一个显着差异是IFNλ刺激驱动树突状细胞趋向于产生CD4 + CD25 + FoxP3 +调节性T细胞的能力，表明可能具有免疫调节作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-28A Mouse ELISA KitRepresentative standard curve for Mouse IL-28A ELISA

技术参数

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IL-28A Mouse ELISA Kit ELISA试剂盒,IL-28A Mouse ELISA Kit/小鼠IL-28A ELISA试剂盒

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