Thrombospondin 1 Human ELISA Kit ELISA试剂盒,Thrombospondin 1 Human ELISA Kit/人血小板反应蛋白1 ELISA试剂盒

2024-10-23

Thrombospondin 1 Human ELISA Kit/人血小板反应蛋白1 ELISA试剂盒

货号:BMS2100,BMS2100TEN

规格:96 Tests,10X96 Tests

价格:6120,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.044 ng/mL

检测范围1.17-75 ng/mL

样本类型/体积

血清 10 μL

血浆 10 μL

操作时间

1 hr 20 min

测试时间

3 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)4.4%
批内变异系数(CV)3.4%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- HRP-共轭检测抗体

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameThrombospondin 1
Protein aliasesGlycoprotein G, Thrombospondin-1, thrombospondin-1p180, thrombospondin-p50

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物HRP
Gene aliasesTHBS, THBS-1, THBS1, TSP, TSP-1, TSP1
Gene ID(Human) 7057
Gene symbolTHBS1
UniProt ID(Human) P07996
The Human Thrombospondin-1 (Hu TSP-1) ELISA quantitates Hu TSP-1 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu TSP-1.人血小板反应蛋白1(人TSP-1)ELISA定量了人血清或血浆中的人TSP-1。 该检测试剂盒将专门识别天然和重组人TSP-1。Principle of the method 方法原理The Human TSP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人TSP-1固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.血小板反应蛋白是血小板α-颗粒的蛋白质。 它由链间二硫键结合的三个相同的亚基组成。 它分泌在血小板活化和聚集的部位,并参与白细胞,成纤维细胞,平滑肌和内皮细胞的趋化性,粘附,增殖和分化过程。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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