IL-25/IL-17E Mouse ELISA Kit ELISA试剂盒,IL-25/IL-17E Mouse ELISA Kit/小鼠IL-25/IL-17E ELISA试剂盒

2024-10-23

IL-25/IL-17E Mouse ELISA Kit/小鼠IL-25/IL-17E ELISA试剂盒

货号:BMS6046,BMS6046TEN

规格:96 Tests,10X96 Tests

价格:6120,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.9 pg/mL

检测范围3.9-250 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

3 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)8.8%
批内变异系数(CV)6.5%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameIL-25
Protein aliasesinterleukin 17E, interleukin-25

物种(Tested)

小鼠

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesIL-17e, Il17e
Gene ID(Mouse) 140806
Gene symbolIl25
The Mouse Interleukin-25/Interleukin 17E (Ms IL-25/IL-17E) ELISA quantitates Ms IL-25/IL-17E in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-25/IL-17E.小鼠白介素25 /白介素17E(小鼠IL-25 / IL-17E)ELISA定量检测小鼠血清,血浆,缓冲液或细胞培养基中的小鼠IL-25 / IL-17E。 该检测试剂盒将专门识别天然和重组小鼠IL-25 / IL-17E。Principle of the method 方法原理The Mouse IL-25/IL-17E solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠IL-25 / IL-17E固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Interleukin 25 (IL-25) is a member of the IL-17 family and is identified as a proinflammatory cytokine which is produced by Th17 effector cells thus enabling the Th17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 also induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. IL-25 itself is activated through IL-23, IL-5, and TGF-beta.白介素25(IL-25)是IL-17家族的成员,被鉴定为促炎细胞因子,由Th17效应细胞产生,因此使Th17亚型能够启动或维持类风湿性关节炎等疾病的自身免疫过程。 IL-25还诱导NF-κB的活化并刺激促炎性趋化因子IL-8的产生。 IL-25本身通过IL-23,IL-5和TGF-beta激活。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

IL-25/IL-17E Mouse ELISA KitRepresentative standard curve for Mouse IL-17E ELISA

技术参数
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