Ghrelin Human ELISA Kit ELISA试剂盒,Ghrelin Human ELISA Kit/人Ghrelin ELISA试剂盒

2024-10-23

Ghrelin Human ELISA Kit/人Ghrelin ELISA试剂盒

货号:BMS2192

规格:96 tests

价格:6120

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

11.8 pg/mL

检测范围15.6-1,000.0 pg/mL

样本类型/体积

血清 25 μL

血浆 25 μL

上清液 25 μL

操作时间

1 hr 20 min

测试时间

3 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)8.5%
批内变异系数(CV)6%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 裂解液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameGhrelin
Protein aliasesAppetite-regulating hormone, Ghrelin, ghrelin, growth hormone secretagogue receptor ligand, Ghrelin-27, Ghrelin-28, ghrelin/obestatin preprohormone, Growth hormone secretagogue, Growth hormone-releasing peptide, In2c-preproghrelin, Motilin-related peptide, Obestatin, prepro-appetite regulatory hormone, preproghrelin, Protein M46

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesGHRL, MTLRP, UNQ524/PRO1066
Gene ID(Human) 51738
Gene symbolGHRL
UniProt ID(Human) Q9UBU3
The Human Ghrelin ELISA quantitates Hu Ghrelin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Ghrelin.人Ghrelin ELISA可以定量人血清,血浆,缓冲液或细胞培养基中的人Ghrelin。 该检测试剂盒将专门识别天然和重组人Ghrelin。Principle of the method 方法原理The Human Ghrelin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人Ghrelin固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an the ligand for the growth hormone secretagogue receptor type 1 and is involved in regulating growth hormone release. Ghrelin has an appeptite-stimulating effect and induces adiposity and stimulates gastric acid secretion. Obestatin may be the ligand for the orphan G protein-coupled receptor GPR39 and is involved in satiety and can have an appetite-reducing effect resulting in decreased food intake.GHRL编码Ghrelin-Obstatin前原蛋白,产生Ghrelin和Obestatin。 Ghrelin是1型生长激素促分泌素受体的配体,参与调节生长激素的释放。 Ghrelin具有刺激胃蛋白酶的作用,并诱导肥胖和刺激胃酸分泌。 肥胖抑制素可能是孤儿G蛋白偶联受体GPR39的配体,与饱腹感有关,可以降低食欲,导致食物摄入减少。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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