Complement C5a Human ELISA Kit ELISA试剂盒,Complement C5a Human ELISA Kit/人补体C5a ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Complement C5a Human ELISA Kit ELISA试剂盒,Complement C5a Human ELISA Kit/人补体C5a ELISA试剂盒

2024-10-23

Complement C5a Human ELISA Kit/人补体C5a ELISA试剂盒

货号：BMS2088,BMS2088TEN

规格：96 Tests,10X96 Tests

价格：6094,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.005 ng/mL
检测范围	0.078-5.0 ng/mL
样本类型/体积	血清 5 μL 血浆 10 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	4 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	4%
批内变异系数（CV）	3.6%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	Complement C5
Protein aliases	anaphylatoxin C5a analog, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, C5a anaphylatoxin, Complement C5, Complement C5 alpha chain, Complement C5 alpha' chain, Complement C5 beta chain, prepro-C5
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	C5, C5a, C5b, C5D, CPAMD4, ECLZB
Gene ID	(Human) 727
Gene symbol	C5
UniProt ID	(Human) P01031

The Human Complement C5a (Hu C5a) ELISA quantitates Hu C5a in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C5a.人补体C5a（人C5a）ELISA定量了人血清，血浆或细胞培养基中的人C5a。该检测试剂盒将只识别天然和重组人C5a。Principle of the method 方法原理The Human C5a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人C5a固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息SRC is a non-receptor kinase involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.SRC是参与真核细胞生长和分化调控的非受体激酶。 SRC与各种细胞表面受体的胞质区域相关联，以促进信号转导，并且还参与细胞骨架的组织，细胞周期的调控和细胞凋亡。在人类中，编码SRC的基因存在于20号染色体上。SRC的活性受两个酪氨酸残基（Tyr 416和Tyr527）的调节，这两个位点的磷酸化作用相反。激酶结构域的激活环中Tyr416的磷酸化上调了酶的活性，而Tyr527的磷酸化则降低了酶的活性。 SRC基因的突变可能与结肠癌的恶性进展有关。迄今为止，已经发现了编码相同SRC蛋白的两个转录物变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

Complement C5a Human ELISA KitRepresentative standard curve for Human C5A ELISA

技术参数

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Complement C5a Human ELISA Kit ELISA试剂盒,Complement C5a Human ELISA Kit/人补体C5a ELISA试剂盒

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