Human IL-29 (IFN lambda 1) ELISA Standard Recombinant Protein /人IL-29(IFN λ 1)ELISA标准重组蛋白
货号:39-8299-65
规格:4 pack
价格:0
产品类型:标准品和抗体对
品牌:Thermo Fisher
物种:人
宿主:其它
抗体亚型:其它
荧光染料:其它
应用 | Assay-Dependent(Ctrl);Assay-Dependent(ELISA) |
种属反应 | Human |
宿主/亚型 | E.coli |
分类 | Recombinant |
类型 | Protein |
偶联物 | Unconjugated |
形式 | Liquid |
纯化类型 | Affinity chromatography |
内含物 | 0.09% sodium azide |
保存条件 | -80°C |
产品详细信息Description: IFN-lambdas are a novel family of interferons which mediate the induction of anti-viral protection in a wide variety of cells. The three members of the IFN-lambda family are lambda1, lambda2, and lambda3, also known as IL-29, IL-28A, and IL-28B, respectively. IFN-lambdas share with type I IFNs an intracellular signaling pathway that drives the expression of a common set of IFN-stimulated genes. IFN-lambdas induce multiple biological activities, including the upregulation of class IMHC gene product expression to levels comparable to those induced by IFN-alphas. IL-28 and IL-29 are tested for anti-viral activity by challenging the human hepatocellular carcinoma cell line HepG2 with infection by EMC (following pretreatment of the cells with cytokine).Consistent with a role in anti-viral protection, the mRNA expression of IFN-lambdas is detectable in cells infected with various viruses. Moreover, monocyte-derived dendritic cells (important producers of IFN-alpha) express IFN-lambda1 mRNA in response to treatment with dsRNA. TLR3 and TLR4 ligands induce IFN-alpha, IFN-beta, IL-28, and IL-29 gene expression in macrophages; this is dependent upon IFN-alpha.IFN-lambdas mediate their anti-viral protection through a class II cytokine receptor complex distinct from that of type I IFNs. This is comprised of two essential receptor proteins, CRF2-12/IFN-lambdaR1, which is unique to IFN-lambdas, and CFR2-4/IL-10R2, which is shared with IL-10, IL-22, and IL-26 receptors. Whereas, the two chains of the type I IFN receptor (IFN-AR1 and IFN-AR2) and IL-10R2 are ubiquitously expressed, IFN-lambdaR1 expression is limited and cell-type dependent. IFN-lambdaR1 is not expressed by monocytes, but is up-regulated during GM-CSF/IL-4 induced differentiation of DCs from human monocytes, yielding iDCs which are fully responsive to IFN-lambda.The IFN-lambdas, IL-28 and IL-29, have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells. IFN-lambda-matured DCs express high levels of class I and II MHC gene products, but low levels of costimulatory molecules, and are able to specifically induce IL-2-dependent proliferation of CD4+CD25+FOXP3+ T cell population with contact dependent suppressive activity on T cells.Applications Reported: Recombinant human IL-29 is biologically active. The intended use of the Recombinant Human IL-29 Single-Use ELISA RSG Standard is as a standard for human IL-29 Ready-SET-Go! ELISA and human IL-29 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the 'top' standard described below.Instructions for use: For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 10 µL of standard solution to 10 mL of assay diluent to prepare the top standard solution (1000 pg/mL).For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 10 µL of standard solution to 10 mL of assay diluent to prepare the top standard solution (1000 pg/mL).Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 µL in 1.0 mL) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.Applications Tested: This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the human IL-29 ELISA pair.Source: E. coli expressed amino acids gly 20-thr 200 of mature human IL-29 accession # NM_172140.Purity: >98% as determined by SDS-PAGE.Storage and handling: For best recovery, quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.靶标信息IFN-lambdas constitute a novel family of interferons that mediate the induction of anti-viral protection in a wide variety of cells. There are three members in this family, namely lambda1, lambda2, and lambda3, which are also known as IL-29, IL-28A, and IL-28B, respectively. Similar to type I IFNs, IFN-lambdas activate an intracellular signaling to promote gene expression. IFN-lambdas mediate their anti-viral protection through a class II cytokine receptor complex composed of two essential receptor proteins, CRF2-12/IFN-lambdaR1, which is unique to IFN-lambdas, and CFR2-4/IL-10R2, which is shared with IL-10, IL-22, and IL-26 receptors. The IFN-lambdas, IL-28 and IL-29, have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells. DCs that differentiate in response to IFN-lambdas express high levels of class I and II MHC gene products, but low levels of costimulatory molecules. Moreover, these cells can specifically induce IL-2-dependent proliferation of CD4+CD25+FOXP3+ T cells with contact-dependent suppressive activity.
©
bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:
https://gibcohyclone.cn/thread-45086.htm