IL-13 Mouse Matched Antibody Pair,IL-13 Mouse Matched Antibody Pair/小鼠IL-13匹配抗体对

2024-10-23

IL-13 Mouse Matched Antibody Pair/小鼠IL-13匹配抗体对

货号:CMC2223

规格:10 x 96 tests

价格:6926

产品类型:标准品和抗体对

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

7.8 pg/mL

检测范围7.8-500 pg/mL

样本类型/体积

血清 100 μL

血浆 100 μL

上清液 100 μL

操作时间

1 hr 30 min

测试时间

24 hr

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 捕获(包被)抗体

- 检测抗体

- 标准品

- 链霉亲和素-HRP

储存温度

2-8℃

Protein nameIL-13
Protein aliasesIL-13, T-cell activation protein P600, Interleukin-13

物种(Tested)

小鼠

试剂盒类型

匹配抗体对

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesIl-13, Il13
Gene ID(Mouse) 16163
Gene symbolIl13
UniProt ID(Mouse) P20109
The Mouse IL-13 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-13. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationIL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据:

IL-13 Mouse Matched Antibody PairRepresentative standard curve for Mouse IL-13 ELISA

技术参数
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