IFN gamma Chicken Matched Antibody Pair/鸡IFN γ 匹配抗体对
货号:CAC1233
规格:10 x 96 tests
价格:6963
产品类型:标准品和抗体对
品牌:Thermo Fisher
物种:其它
宿主:其它
抗体亚型:其它
荧光染料:其它
灵敏度 | <15.6 pg/mL |
检测范围 | 15.6-1000 pg/mL |
样本类型/体积 | 血清 100 μL 血浆 100 μL 上清液 100 μL |
操作时间 | 1 hr 30 min |
测试时间 | 24 hr |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 x 96 tests |
组份 | - 捕获(包被)抗体 - 检测抗体 - 标准品 - 链霉亲和素-HRP |
储存温度 | 2-8℃ |
Protein name | IFN gamma |
Protein aliases | IFN-gamma, Interferon gamma, interferon-gama, Interferon-gamma level |
物种(Tested) | 鸡 |
试剂盒类型 | 匹配抗体对 |
标记(染料) | HRP |
检测抗体偶联物 | Biotin |
Gene aliases | IFNG |
Gene ID | (Chicken) 396054 |
Gene symbol | IFNG |
UniProt ID | (Chicken) P49708 |
The Chicken Interferon gamma Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of chicken IFNγ. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationIFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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