IL-6 Human Matched Antibody Pair/人IL-6匹配抗体对

货号：BMS213-2MST

规格：10 x 96 tests

价格：6614

产品类型：标准品和抗体对

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	1.56 pg/mL
检测范围	1.56-100 pg/mL
样本类型/体积	血清 100 μL 血浆 100 μL 上清液 100 μL
操作时间	1 hr 10 min
测试时间	24 hr
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 捕获（包被）抗体 - 检测抗体 - 标准品 - SAV-HRP 偶联
储存温度	-20°C
Protein name	IL-6
Protein aliases	B-cell differentiation factor, B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6, Interferon beta-2, interleukin BSF-2, Interleukin-6
物种（Tested）	人
试剂盒类型	匹配抗体对
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6, IL6
Gene ID	(Human) 3569
Gene symbol	IL6
UniProt ID	(Human) P05231

The Human IL-6 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-6. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationInterleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

技术参数

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