IL-6 Bovine Uncoated ELISA Kit ELISA试剂盒,IL-6 Bovine Uncoated ELISA Kit/牛IL-6未包被ELISA试剂盒

2024-10-23

IL-6 Bovine Uncoated ELISA Kit/牛IL-6未包被ELISA试剂盒

货号:ESS0029

规格:5 x 96 tests

价格:7267

产品类型:自定义ELISA

品牌:Thermo Fisher

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

<78.1 pg/mL

检测范围78.1-5000 pg/mL

样本类型/体积

上清液 100 μL

血清 100 μL

操作时间

24 hr

测试时间

2 hr 50 min

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5 x 96 tests

组份

- 包被抗体

- 冻干重组标准品

- 检测抗体

- 链霉亲和素-HRP

- 底物溶液

- 即用型终止液,br> 0.16M硫酸

- 分析证书:特定批次的抗体和标准品稀释说明

储存温度

2-8℃

Protein nameIL-6
Protein aliasesIL-6, Interleukin-6

物种(Tested)

试剂盒类型

Uncoated ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesBOS_4106, IL6
Gene ID(Bovine) 280826
Gene symbolIL6
UniProt ID

(Bovine) P26892

The Bovine IL-6 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of bovine IL-6.牛IL-6非包被ELISA试剂盒包含预匹配的抗体对和试剂,用于进行定量酶联免疫吸附测定(ELISA),以检测和定量牛IL-6的蛋白质水平。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA用于测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被在微孔板孔的底部,这是一个过夜的过程。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.白介素6(IL-6)是最初在RNA刺激的成纤维细胞细胞培养基中发现的多功能26 kD蛋白。 IL-6直接参与感染和细胞损伤后发生的反应,在调节急性期反应中作为IL-1和TNF-a很重要。据报道IL-6由成纤维细胞,活化的T细胞,活化的单核细胞或巨噬细胞和内皮细胞产生。 IL-6作用于多种细胞,包括髓样祖细胞,T细胞,B细胞和肝细胞。另外,IL-6似乎在T淋巴细胞的增殖中与IL-2相互作用。 IL-6增强了IL-3对多能造血祖细胞的增殖作用。 IL-6在B细胞向浆细胞的分化中起关键作用,并且是浆细胞瘤和骨髓瘤的有效生长因子。 IL-6是一种非常有用的培养补充剂,可用于产生大量产生抗体的杂交瘤。 IL-6主要在急性和慢性炎症部位产生,被分泌到血清中并通过白介素6受体α诱导转录炎症反应。 IL-6的功能涉及多种与炎症相关的疾病状态,包括糖尿病和全身性青少年类风湿性关节炎。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

IL-6 Bovine Uncoated ELISA KitRepresentative standard curve for bovine IL-6 ELISA

技术参数
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