CD209b (SIGN-R1) Monoclonal Antibody (eBio22D1 (22D1)), eBioscience

货号：14-2093-81,14-2093-82

规格：50 µg,100 µg

价格：1789,1833

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：eBio22D1 (22D1)

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1 µg/test （Flow）；Assay-Dependent（ELISA)；Assay-Dependent(FN)；Assay-Dependent(IHC (F))；Assay-Dependent(IP)；(IF)；(IHC)；(ICC)

产品详细信息Description: The eBio22D1 monoclonal antibody reacts with mouse SIGNR1 (CD209b). SIGNR1 is a type II transmembrane C-type lectin that was identified in a search for mouse homologues of human DC-SIGN. It is expressed at high levels in splenic marginal zone macrophages and lymph node medullary macrophages, where it functions to uptake dextran polysaccharides, including the capsular polysaccharide of Streptococcus pneumoniae. It has also been demonstrated that SIGNR1 physically associates with TLR4/MD2, and it has been suggested that this association plays a role in recognition of LPS. Furthermore, recently it has been shown that SIGNR1 deficient mice have a defect in catabolism of the complement component C3, and that SIGNR1 binds directly to the complement C1 subcomponent, C1q to assemble a non-conventional C3 convertase. The eBio22D1 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.Applications Reported: This eBio22D1 (22D1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistology staining of frozen tissue sections, and ELISA. (Please use Functional Grade purified eBio22D1 (22D1), cat. 16-2093, in functional assays.).Applications Tested: This eBio22D1 (22D1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息SIGNR1 is a type II transmembrane C-type lectin that was identified in a search for mouse homologues of human DC-SIGN. It is expressed at high levels in splenic marginal zone macrophages and lymph node medullary macrophages, where it functions to uptake dextran polysaccharides, including the capsular polysaccharide of Streptococcus pneumoniae. It has also been demonstrated that SIGNR1 physically associates with TLR4/MD2, and it has been suggested that this association plays a role in recognitionof LPS. Furthermore, recently it has been shown that SIGNR1 deficient mice have a defect in catabolism of the complement component C3, and that SIGNR1 binds directly to the complement C1 subcomponent, C1q to assemble a non-conventional C3 convertase.

数据

CD209b (SIGN-R1) Antibody (14-2093-81) in FlowStaining of C57BL/6 splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.5 µg of Anti-Mouse CD209b (SIGN-R1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG PE (Product # 12-4112-83). Cells in the lymphocyte and monocyte gates were used for analysis, and events displayed are gated on CD11c+ cells. CD209b (SIGN-R1) Antibody (14-2093-81)Immunity and ageing : I and A 2015 -Figure 8 Marginal zone B cell regions were narrowed in Ziz2 KO mice. (A-B) Splenic sections were stained with anti-CD1d (Magenta), anti-CD169 (Green) (B) , and anti-SIGN-R1(Blue) antibodies (A) Three mice per group were used. Between one and eight follicles per mouse (per section) were captured. All mice were 10 weeks old. Scale bars: 100 mum. (C) The CD1d-positive region outside CD169-positive cells was significantly narrower in Ziz2 KO mice than in the other groups. 2KO: Ziz2 KO. 3KO: Ziz3 KO. **: P < 0.01 ***: P < 0.0001 N = 7-15.

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流式抗体

参考文献：

1.Proceedings of the National Academy of Sciences of the United States of AmericaC-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells.2.VirologyPrion pathogenesis is unaltered following down-regulation of SIGN-R1.3.Immunity and ageing : I and AThe immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.4.ImmunologyPeripheral prion disease pathogenesis is unaltered in the absence of sialoadhesin (Siglec-1/CD169).5.Journal of immunology (Baltimore, Md. : 1950)Alterations in marginal zone macrophages and marginal zone B cells in old mice.

技术参数

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