| 产品介绍 | | |
| Rat anti Mouse CD34 antibody, clone MEC14.7recognizes the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies. |
| 产品详情 | | |
Target Species | Mouse |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Immunogen | T-end.1, a pMT transformed endothelial cell line. |
| Approx. Protein Concentrations | Pack Size: 0.1 mg, 50 µg IgG concentration 0.1 mg/ml Pack Size: 0.5 mg IgG concentration 0.5 mg/ml |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/5 | NeatPack Size: 0.1 mg1/10Pack Size: 0.5 mg |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
|
| 数据 |
| Staining of mouse bone marrow cells with Rat anti Mouse CD34:FITC(MCA1825F) |
| 同型 |
| Description | Product Code | Applications | Pack Size |
Rat IgG2a Negative Control:FITC | MCA1212F | F | 100 Tests |
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| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | F | 50 Test |
| Human Seroblock | BUF070B | F | 200 Test |
| 文献 |
| 1. Winding, B.et al.(2002) Synthetic matrix metalloproteinase inhibitors inhibit growth of established breast cancer osteolytic lesions and prolong survival in mice.Clin Cancer Res. 8 (6): 1932-9.2. Nguyen, L.et al.(2012) Spatial morphological and molecular differences within solid tumors may contribute to the failure of vascular disruptive agent treatments.BMC Cancer. 12: 522.3. Morison, N.B.et al.(2007) The long-term actions of etonogestrel and levonorgestrel on decidualized and non-decidualized endometrium in a mouse model mimic some effects of progestogen-only contraceptives in women.Reproduction. 133: 309-21.4. Chen, L.et al.(2010) Roles of tetrahydrobiopterin in promoting tumor angiogenesis.Am J Pathol. 177: 2671-80.5. Ager, E.I.et al.(2010) Targeting the angiotensin II type 2 receptor (AT2R) in colorectal liver metastases.Cancer Cell Int. 10: 196. Chabot, S.et al.(2011) A novel antiangiogenic and vascular normalization therapy targeted against human CD160 receptor.J Exp Med. 208: 973-86.7. Chen, J.et al.(2011) Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in cerebral ischemic damage.Am J Physiol Endocrinol Metab. 2011 Jul;301(1):E62-71.8. Chen, J.et al.(2012) Transfusion of CXCR4-primed endothelial progenitor cells reduces cerebral ischemic damage and promotes repair in db/db diabetic mice.PLoS One. 7 (11): e50105.9. Ouji, Y. & Yoshikawa, M. (2016) Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro.Methods Mol Biol. 1516: 279-88.10. Nguyen, L.et al.(2016) Vascular disruptive agent OXi4503 and anti-angiogenic agent Sunitinib combination treatment prolong survival of mice with CRC liver metastasis.BMC Cancer. 16 (1): 533.11. Vávrová, J.et al.(2012) Irradiated stem cells and ageing of the haematopoietic system.Radiat Environ Biophys. 51 (2): 205-13.12. DaCosta, P.L.N.et al.(2018) The kallikrein-Kinin system modulates the progression of colorectal liver metastases in a mouse model.BMC Cancer. 18 (1): 382.13. Danielyan, L.et al.. (2020) Cell motility and migration as determinants of stem cell efficacyEBioMedicine. 60:102989.14. Rackham, C.L.et al.(2013) Maintenance of islet morphology is beneficial for transplantation outcome in diabetic mice.PLoS One. 8 (2): e57844.15. Fruchon, S.et al.(2012) Involvement of the Syk-mTOR pathway in follicular lymphoma cell invasion and angiogenesis.Leukemia. 26 (4): 795-805.16. Zhu, C.et al.(2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.J Neuroinflammation. 17 (1): 85. |
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