Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience/Galectin 3单克隆抗体（eBioM3 / 38（M3 / 38））

货号：14-5301-81,14-5301-82,14-5301-85

规格：50 µg ,100 µg ,500 µg

价格：1130,2257,3674

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：其它

抗体亚型：IgG2a, kappa

克隆号：eBioM3/38 (M3/38)

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.25 µg/test（Flow）；Assay-Dependent（ELISA)；Assay-Dependent (IHC (F)) ；Assay-Dependent(IHC (P))；Assay-Dependent；(IP)；Assay-Dependent(WB)；(IHC)；(IF)

产品详细信息Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, immunohistology staining of paraffin embedded tissue sections, and ELISA.Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse PECs (thioglycollated elicied peritioneal cells). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation ofmast cells.

数据

Galectin 3 Antibody (14-5301-81) in FlowStaining of mouse thioglycolate-induced peritoneal exudate cells (PECs) with 0.25 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.25 µg of Anti-Human/Mouse Galectin-3 Purified (filled histogram) followed by F (ab')2 Anti-Rat IgG APC (Product # 17-4822-82). Total viable cells were used for analysis. Galectin 3 Antibody (14-5301-81)Cell death and disease 2016 -Figure 2 Gal3 positive CSC subset displays increased stemness characteristics. ( a ) ALDH activity was evaluated by flow cytometric analysis in Gal3 positive CSC (in red) and Gal3 negative CSC (in green). ALDH negative fractions are illustrated in gray. ( b ) Sphere formation ability (SFA) was determined. Gal3 positive CSC (red) accounted for significantly more spheres than Gal3 negative CSC (green). ( c ) TRAIL-resistance in Gal3 positive /Gal3 negative colon cancer CSC subsets was determined by APO-BRDU assay. Apoptotic cells are displayed as percentage of total cell count. ( d ) TRAIL-resistance in Gal3 positive /Gal3 negative CSC pancreatic cancer subsets. All experiments were performed in triplicate (see text for details). P <0.05 (*), P <0.01 (**), and P <0.001 (***) Galectin 3 Antibody (14-5301-81)Frontiers in cellular neuroscience 2015 -FIGURE 2 Embryonic microglial cell population is poorly immunoreactive to the Mac-2/Galectin-3 antibody after single and double injection of poly (I:C). (A-F1) Coronal sections of embryonic brains, with cell nucleus staining in blue (DAPI) and microglial (CX3CR1-eGFP) cells in green. Immunohistochemical staining using a Mac-2 antibody (red) showed that at E17.5 almost no microglial cells in the cortex were immunoreactive for Mac-2 (A2) after injection with saline. At E11.5 (B2) and E17.5 (C2,E2) in the cortex and E17.5 hippocampal area (D2,F2) there was no increased percentage of microglial cells expressing the activation marker after poly (I:C) challenge compared to control. White square indicates the location of the cells in the tissue showed in the inset; * indicates a Mac-2 positive eGFP cell. Examples of one control brain area and poly (I:C) group only as they were not significantly different. Scale bar = 100 mum and for insets = 20 mum.

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参考文献：

1.PloS oneTime-course analysis of cardiac and serum galectin-3 in viral myocarditis after an encephalomyocarditis virus inoculation. 2.JCI insight Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis.3.Cell death and diseaseCell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics.4.Cell death and diseaseCell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics.5.PLoS pathogensRhadinovirus host entry by co-operative infection.

技术参数

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