IgM Monoclonal Antibody (II/41), eBioscience/IgM单克隆抗体（II / 41）

货号：14-5790-81,14-5790-82,14-5790-85

规格：50 µg,100 µg,500 µg

价格：1572,1399,2122

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：II/41

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1 µg/test（Flow）；Assay-Dependent（ELISA)；Assay-Dependent(IHC (F))；(TCA)；(IHC)；(IF)

产品详细信息Description: The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: The II/41 antibody has been reported for use inflow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA.Applications Tested: The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspension. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (14-5790-81) in FlowStaining of C57BL/6 splenocytes with 0.5 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Mouse IgM Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis. IgM Antibody (14-5790-81)Cell regeneration (London, England) 2018 -Fig. 2 Flow cytometry analysis of the purity of sorted Ter119 - Mac1 - CD3 - CD4 - CD8 - B220 + CD19 + CD93 + IgM - pro/pre-B cells . Bone marrow nucleated cells were first incubated with biotin-conjugated anti-B220 antibody and then enriched by streptavidin microbeads using AutoMACS Pro. The pro/pre-B cells were further sorted from the B220 enriched cells by defined markers. Flow cytometry analysis confirmed the purities of B220 enriched cells (top) and sorted pro/pre-B cells (bottom). IgM Antibody (14-5790-81)Journal of immunology (Baltimore, Md. : 1950) 2012 -Published figure using IgM monoclonal antibody (Product # 14-5790-81)

推荐产品：

流式抗体

参考文献：

1.Cell regeneration (London, England)A protocol for generating induced T cells by reprogramming B cellsinvivo.2.Nature communicationsPI3Kδ hyper-activation promotes development of Bcells that exacerbate Streptococcus pneumoniae infection in an antibody-independent manner.3. Molecular medicine reportsAnti‑IL‑39 (IL‑23p19/Ebi3) polyclonal antibodies ameliorate autoimmune symptoms in lupus‑like mice.4. AutophagyB cell autophagy mediates TLR7-dependent autoimmunity and inflammation.5. ImmunityNuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-45192.htm